Assay for Flavins in Rat Heart Mitochondria by High Pressure Liquid Chromatography

The high pressure liquid chromatographic analysis of riboflavin, FMN and FAD in isolated heart mitochondria was described. The flavin content in rat heart mitochondria were : 0.67±0.15n moles FAD per mg protein, 0.13±0.13 n moles FMN and 0.008±0.004 n moles free riboflavin. The preparation of mitoch...

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Veröffentlicht in:	Kurume medical journal 1986/08/25, Vol.33(1), pp.1-6
Hauptverfasser:	OGURA, RYOHEI, HINO, YUJI, YOON, SUNG BOO, KAGIYAMA, AKIHIRO, IWAHASHI, HIROSHI, HIDAKA, TOSHIHIRO
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Chromatography, High Pressure Liquid determination of flavin extraction of flavin Flavins - analysis high pressure liquid chromatography isolation of heart mitochondria Microscopy, Electron Mitochondria, Heart - analysis Mitochondria, Heart - ultrastructure Rats respiratory responses
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Zusammenfassung:	The high pressure liquid chromatographic analysis of riboflavin, FMN and FAD in isolated heart mitochondria was described. The flavin content in rat heart mitochondria were : 0.67±0.15n moles FAD per mg protein, 0.13±0.13 n moles FMN and 0.008±0.004 n moles free riboflavin. The preparation of mitochondria from rat heart which was used to determine the flavin concentrations was also described.
ISSN:	0023-5679 1881-2090
DOI:	10.2739/kurumemedj.33.1