PROPOSAL OF STANDARDIZED METHODS AND REFERENCE FOR ASSAYING RECOMBINANT HUMAN TUMOR NECROSIS FACTOR

PROPOSAL OF STANDARDIZED METHODS AND REFERENCE FOR ASSAYING RECOMBINANT HUMAN TUMOR NECROSIS FACTOR

Two assay methods for recombinant human tumor necrosis factor (rH-TNF) were developed, one a biological L-cell assay and the other an enzyme-linked immunosorbent assay. The accuracy and reproducibility of each and the correlation between the two were studied. As a result of this investigation, the t...

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Veröffentlicht in:Japanese Journal of Medical Science and Biology 1986, Vol.39(3), pp.105-118
Hauptverfasser: YAMAZAKI, Shudo, ONISHI, Eiko, ENAMI, Kazue, NATORI, Katsuro, KOHASE, Masayoshi, SAKAMOTO, Hajime, TANOUCHI, Masatoshi, HAYASHI, Hiroshi
Format: Artikel
Sprache:eng
Schlagworte:
Antibodies, Monoclonal
Biological Assay
Cell Survival - drug effects
Enzyme-Linked Immunosorbent Assay
Freeze Drying
Glycoproteins - immunology
Glycoproteins - pharmacology
Glycoproteins - standards
Humans
L Cells (Cell Line)
Tumor Necrosis Factor-alpha
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Zusammenfassung:Two assay methods for recombinant human tumor necrosis factor (rH-TNF) were developed, one a biological L-cell assay and the other an enzyme-linked immunosorbent assay. The accuracy and reproducibility of each and the correlation between the two were studied. As a result of this investigation, the two assay methods were found appropriate for standardization of rH-TNF. A freeze-dried reference was prepared, and examination of its potency and stability showed it to be suitable for use as a reference standard for rH-TNF assays.
ISSN:0021-5112
1884-2828
DOI:10.7883/yoken1952.39.105