A sulfatide-reactive human monoclonal antibody obtained from a multiple sclerosis patient selectively binds to the surface of oligodendrocytes

In a previous paper, we described the production of a sulfatide-reactive IgM antibody-secreting B cell line that was obtained by Epstein-Barr virus transformation of peripheral B cells from a patient with multiple sclerosis (MS) (Uhlig and Dernick, 1989). In the present study, we demonstrate that th...

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Veröffentlicht in:	Journal of neuroimmunology 1995-02, Vol.56 (2), p.191-200
Hauptverfasser:	Kirschning, Eckhard, Rutter, Gabriel, Uhlig, Hans, Dernick, Rudolf
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal - immunology Cells, Cultured Cross Reactions Galactocerebroside Human monoclonal autoantibodies Multiple sclerosis Multiple Sclerosis - immunology Myelin Proteins - immunology Oligodendrocytes Oligodendroglia - immunology Rats Rats, Inbred Lew Sulfatide Sulfoglycosphingolipids - immunology
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Zusammenfassung:	In a previous paper, we described the production of a sulfatide-reactive IgM antibody-secreting B cell line that was obtained by Epstein-Barr virus transformation of peripheral B cells from a patient with multiple sclerosis (MS) (Uhlig and Dernick, 1989). In the present study, we demonstrate that this human monoclonal antibody (humAb) DS1F8 selectively binds to the surface of living oligodendrocytes in mixed brain cell cultures of newborn rats. Since a mouse mAb reactive with sulfatide was shown to inhibit oligodendrocyte progenitor differentiation, autoantibodies with binding specificities similar to DS1F8 could play a role in the demyelinating process in the CNS.
ISSN:	0165-5728 1872-8421
DOI:	10.1016/0165-5728(94)00150-M