Preliminary serological characterization of bovine viral diarrhoea virus strains using monoclonal antibodies

Five monoclonal antibodies against the bovine viral diarrhoea (BVD) viral strain NADL were isolated and characterized by an indirect immunofluorescence assay. Extensive cross-reactions were detected when the antibodies were tested with 12 heterologous BVD and four hog cholera (HC) viral strains. One...

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Veröffentlicht in:Veterinary microbiology 1986-09, Vol.12 (3), p.195-200
Hauptverfasser: Peters, W., Greiser-Wilke, I., Moennig, V., Liess, B.
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container_issue 3
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container_title Veterinary microbiology
container_volume 12
creator Peters, W.
Greiser-Wilke, I.
Moennig, V.
Liess, B.
description Five monoclonal antibodies against the bovine viral diarrhoea (BVD) viral strain NADL were isolated and characterized by an indirect immunofluorescence assay. Extensive cross-reactions were detected when the antibodies were tested with 12 heterologous BVD and four hog cholera (HC) viral strains. One antibody reacted with all strains tested. Two antibodies were specific for cytopathogenic BVD viruses, but failed to react with HC virus. The other antibodies reacted to varying degrees with BVD and HC viral strains.
doi_str_mv 10.1016/0378-1135(86)90048-9
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ispartof Veterinary microbiology, 1986-09, Vol.12 (3), p.195-200
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source MEDLINE; ScienceDirect Journals (5 years ago - present)
subjects Animals
Antibodies, Monoclonal - immunology
Antibodies, Monoclonal - isolation & purification
Antibodies, Viral - immunology
Antibodies, Viral - isolation & purification
Antibody Specificity
Antigens, Viral - immunology
Classical Swine Fever Virus - immunology
Cross Reactions
Diarrhea Viruses, Bovine Viral - immunology
Electrophoresis, Polyacrylamide Gel
Fluorescent Antibody Technique
Immunoglobulin G - immunology
Immunoglobulin G - isolation & purification
Mice
Mice, Inbred BALB C
Pestivirus - immunology
title Preliminary serological characterization of bovine viral diarrhoea virus strains using monoclonal antibodies
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