Preliminary serological characterization of bovine viral diarrhoea virus strains using monoclonal antibodies

Five monoclonal antibodies against the bovine viral diarrhoea (BVD) viral strain NADL were isolated and characterized by an indirect immunofluorescence assay. Extensive cross-reactions were detected when the antibodies were tested with 12 heterologous BVD and four hog cholera (HC) viral strains. One...

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Veröffentlicht in:	Veterinary microbiology 1986-09, Vol.12 (3), p.195-200
Hauptverfasser:	Peters, W., Greiser-Wilke, I., Moennig, V., Liess, B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal - immunology Antibodies, Monoclonal - isolation & purification Antibodies, Viral - immunology Antibodies, Viral - isolation & purification Antibody Specificity Antigens, Viral - immunology Classical Swine Fever Virus - immunology Cross Reactions Diarrhea Viruses, Bovine Viral - immunology Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Immunoglobulin G - immunology Immunoglobulin G - isolation & purification Mice Mice, Inbred BALB C Pestivirus - immunology
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Zusammenfassung:	Five monoclonal antibodies against the bovine viral diarrhoea (BVD) viral strain NADL were isolated and characterized by an indirect immunofluorescence assay. Extensive cross-reactions were detected when the antibodies were tested with 12 heterologous BVD and four hog cholera (HC) viral strains. One antibody reacted with all strains tested. Two antibodies were specific for cytopathogenic BVD viruses, but failed to react with HC virus. The other antibodies reacted to varying degrees with BVD and HC viral strains.
ISSN:	0378-1135 1873-2542
DOI:	10.1016/0378-1135(86)90048-9