Squamous cell carcinoma antigen is a potent inhibitor of cysteine proteinase cathepsin L

A squamous cell carcinoma antigen (SSCA), which is a member of the serpin family of proteinase inhibitors, was purified from sera of cancer patients. It did not inhibit serine proteinases. However, it non-competitively inhibited human cathepsin L with a K i of 0.064 nM, but not cathepsins B and H am...

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Veröffentlicht in:	FEBS letters 1995-02, Vol.359 (1), p.78-80
Hauptverfasser:	Takeda, Atsushi, Yamamoto, Takako, Nakamura, Yoshiko, Takahashi, Tadahito, Hibino, Toshihiko
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigens, Neoplasm - blood Antigens, Neoplasm - pharmacology Boc, t-butyloxycarbonyl Carcinoma, Squamous Cell - immunology Cathepsin L Cathepsins - antagonists & inhibitors Chromatography, Affinity Cysteine Endopeptidases DTT, dithiothreitol Endopeptidases Female FPLC, fast protein liquid chromatography HPLC, high performance liquid chromatography Humans Kinetics Lung Neoplasms - immunology MCA, 4-methylcoumaryl-7-amide Protease Inhibitors - pharmacology Proteinase inhibitor SCCA, squamous cell carcinoma antigen SDS, sodium dodecyl sulfate Serpin Serpins Squamous cell carcinoma antigen (SCCA) Suc(OMe), N-methoxysuccinyl Suc, succinyl Swine Uterine Cervical Neoplasms - immunology Z, carbobenzoxy
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Zusammenfassung:	A squamous cell carcinoma antigen (SSCA), which is a member of the serpin family of proteinase inhibitors, was purified from sera of cancer patients. It did not inhibit serine proteinases. However, it non-competitively inhibited human cathepsin L with a K i of 0.064 nM, but not cathepsins B and H among cysteine proteinases. These results indicated that SCCA is a non-functional serpin that inhibits cathepsin L in cancer cells.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(94)01456-B