Absence of p350 Subunit of DNA-Activated Protein Kinase from a Radiosensitive Human Cell Line

The radiosensitive rodent mutant cell line xrs-5 is defective in DNA double-strand break repair and lacks the Ku component of the DNA-activated protein kinase, DNA-PK. Here radiosensitive human cell lines were analyzed for DNA-PK activity and for the presence of related proteins. The radiosensitive...

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Veröffentlicht in:	Science 1995-02, Vol.267 (5201), p.1183-1185
Hauptverfasser:	Lees-Miller, Susan P., Godbout, Roseline, Chan, Doug W., Weinfeld, Michael, Day, Rufus S., Barron, Geraldine M., Allalunis-Turner, Joan
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Antigens, Nuclear Biochemistry Biological and medical sciences BIOLOGICAL REPAIR BIOLOGY AND MEDICINE, APPLIED STUDIES Cell extracts Cell Line, Transformed Cell lines CHO Cells Cricetinae Deoxyribonucleic acid DNA DNA - metabolism DNA Helicases DNA Repair DNA-Activated Protein Kinase DNA-Binding Proteins - analysis ECOLOGICAL CONCENTRATION Fundamental and applied biological sciences. Psychology Gamma Rays Gels Genes GENETIC RADIATION EFFECTS Glioma HeLa cells HUMAN POPULATIONS Humans Ionizing radiation Ku Autoantigen MAN Molecular and cellular biology Molecular genetics Molecular Sequence Data Mutagenesis. Repair Mutation Nuclear Proteins - analysis p350 subunit PHOSPHOTRANSFERASES protein kinase Protein kinases Protein-Serine-Threonine Kinases - metabolism Radiation Tolerance RNA Rodents Transcripts (Written Records) Tumor Cells, Cultured X RADIATION
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Beschreibung
Zusammenfassung:	The radiosensitive rodent mutant cell line xrs-5 is defective in DNA double-strand break repair and lacks the Ku component of the DNA-activated protein kinase, DNA-PK. Here radiosensitive human cell lines were analyzed for DNA-PK activity and for the presence of related proteins. The radiosensitive human malignant glioma M059J cell line was found to be defective in DNA double-strand break repair, but fails to express the p350 subunit of DNA-PK. These results suggest that DNA-PK kinase activity is involved in DNA double-strand break repair.
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.7855602