Quantitation of Vasopressin mRNA and Oxytocin mRNA in Hypothalamic Nuclei by Solution Hybridization Assays

Quantitation of Vasopressin mRNA and Oxytocin mRNA in Hypothalamic Nuclei by Solution Hybridization Assays

: Concentrations of vasopressin (VP) precursor and oxytocin (OT) precursor mRNA were measured in magno‐cellular cell groups of the rat hypothalamus by newly developed solution hybridization assays. The assays employed single‐stranded 35S‐labeled VP‐specific and OT‐specific DNA probes that were prepa...

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Veröffentlicht in:Journal of neurochemistry 1986-12, Vol.47 (6), p.1814-1821
Hauptverfasser: Burbach, J. Peter H., Tol, Hubert H. M., Bakkus, Marleen H. C., Schmale, Hartwig, Ivell, Richard
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Biological and medical sciences
Central nervous system
Central neurotransmission. Neuromudulation. Pathways and receptors
Fundamental and applied biological sciences. Psychology
Hypothalamus - analysis
Hypothalamus - metabolism
Male
Neuropeptide gene regulation
Nucleic Acid Hybridization
Oxytocin - biosynthesis
Oxytocin - genetics
Oxytocin mRNA
Paraventricular Hypothalamic Nucleus - analysis
Paraventricular Hypothalamic Nucleus - metabolism
Paraventricular nucleus
Rats
Rats, Inbred Strains
RNA, Messenger - analysis
Solution hybridization assay
Supraoptic nucleus
Vasopressin mRNA
Vasopressins - biosynthesis
Vasopressins - genetics
Vertebrates: nervous system and sense organs
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Zusammenfassung:: Concentrations of vasopressin (VP) precursor and oxytocin (OT) precursor mRNA were measured in magno‐cellular cell groups of the rat hypothalamus by newly developed solution hybridization assays. The assays employed single‐stranded 35S‐labeled VP‐specific and OT‐specific DNA probes that were prepared by primer extension on re‐combinant M13 DNA templates. Solution hybridization assays were standardized by known amounts of cloned DNA. The detection limit was
ISSN:0022-3042
1471-4159
DOI:10.1111/j.1471-4159.1986.tb13093.x