Identification of the novel HLA-DPB15801 allele detected by sequenced based typing

Within the framework of HLA-DPB typing of the Fourth Asia-Oceania Histocompatibility workshop (4AOH) we have typed the A, B, and E panels representing 101 samples (L. N. Daly, personal communication). Sequenced based typing (SBT) was used as the method for typing described by Versluis and co-workers...

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Veröffentlicht in:	Immunogenetics 1995-01, Vol.41 (2-3), p.173-173
Hauptverfasser:	Versluis, L F, Daly, L N, Degli-Eposti, M A, van der Zwan, A W, Dawkins, R L, Tilanus, M G
Format:	Artikel
Sprache:	eng
Schlagworte:	Alleles AMINO ACID SEQUENCE Base Sequence BIOLOGY AND MEDICINE, BASIC STUDIES DNA SEQUENCING DNA-CLONING Exons - genetics GENES Heterozygote HISTOCOMPATIBILITY COMPLEX Histocompatibility Testing - methods HLA-DP Antigens - genetics HLA-DP beta-Chains Humans Molecular Sequence Data NUCLEOTIDES POLYMERASE CHAIN REACTION Sequence Analysis, DNA
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Zusammenfassung:	Within the framework of HLA-DPB typing of the Fourth Asia-Oceania Histocompatibility workshop (4AOH) we have typed the A, B, and E panels representing 101 samples (L. N. Daly, personal communication). Sequenced based typing (SBT) was used as the method for typing described by Versluis and co-workers (1993), but the sequencing chemistry was modified; in this study we have used the Sequenase enzyme instead of the thermal stable Taq enzyme. Sequences obtained were compared to a database containing all known 55 HLA-DPB1 alleles. One sample (4AOH100107X) showed a new heterozygous sequence, indicating the presence of a new allele.
ISSN:	0093-7711
DOI:	10.1007/BF00182337