Kinetic properties of ovine adipose tissue fructose-1,6-bisphosphatase

The presence of FBPase was confirmed in both human and ovine white adipose tissue in metabolically significant amounts. The partially purified enzyme from ovine adipose tissue exhibited kinetic properties very similar to other mammalian FBPases (pH optimum of 7.5, absolute requirement for divalent m...

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Veröffentlicht in:	Comparative biochemistry and physiology. B, Comparative biochemistry Comparative biochemistry, 1994-10, Vol.109 (2), p.443-450
Hauptverfasser:	Koekemoer, T.C., Stanton, L.-A., Oelofsen, W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adipose Tissue - enzymology AMP inhibition Animals F-2,6-P 2 inhibition Fructose-1,6-bisphosphatase Fructose-Bisphosphatase - metabolism Gluconeogenesis Human adipose tissue Humans Hydroxylapatite chromatography Kinetics Metal ion requirement Nongluconeogenic tissue Ovine adipose tissue Rabbits Rats Sheep - metabolism
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Beschreibung
Zusammenfassung:	The presence of FBPase was confirmed in both human and ovine white adipose tissue in metabolically significant amounts. The partially purified enzyme from ovine adipose tissue exhibited kinetic properties very similar to other mammalian FBPases (pH optimum of 7.5, absolute requirement for divalent metal ions and strong inhibition by both AMP and F-2,6-P 2). The micromolar S 0.5 value obtained suggests that the enzyme may be of physiological significance.
ISSN:	0305-0491 1096-4959
DOI:	10.1016/0305-0491(94)90027-2