New modified substrates for discriminating between human DNA polymerases α and ϵ

Two 2′-deoxynucleoside 5′-α-methylenephosphonyl-β,γ-diphosphates were synthesized. They were incorporated into the DNA chain by DNA polymerase α from human placenta. Meanwhile, they were not recognized by DNA polymerases ϵ and β of the same origin as well as by reverse transcriptases from human immu...

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Veröffentlicht in:	FEBS letters 1995-01, Vol.357 (1), p.23-26
Hauptverfasser:	Jasko, Maxim V., Semizarov, Dmitry G., Victorova, Lyubov S., Mozzherin, Dmitry Ju, Krayevsky, Alexander A., Kukhanova, Marina K.
Format:	Artikel
Sprache:	eng
Schlagworte:	2′,3′-dideoxynucleoside 5′-triphosphate 2′-deoxynucleoside 5′-monophosphate 2′-deoxynucleoside 5′-triphosphate AIDS/HIV AMV avian myeloblastosis virus Avian Myeloblastosis Virus - enzymology Base Sequence d2NTP DNA - metabolism DNA polymerase DNA polymerase I Klenow fragment from E. coli DNA Polymerase II - metabolism DNA-Directed DNA Polymerase - metabolism dNMP dNTP HIV HIV - enzymology human immunodeficiency virus Humans KFr Modified nucleoside 5′-triphosphate Molecular Sequence Data Nucleotides - metabolism Reverse transcriptase RNA-Directed DNA Polymerase - metabolism Substrate Specificity TdT Terminal deoxynucleotidyl transferase
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Zusammenfassung:	Two 2′-deoxynucleoside 5′-α-methylenephosphonyl-β,γ-diphosphates were synthesized. They were incorporated into the DNA chain by DNA polymerase α from human placenta. Meanwhile, they were not recognized by DNA polymerases ϵ and β of the same origin as well as by reverse transcriptases from human immunodeficiency virus and avian myeloblastosis virus.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(94)01319-V