Viewing of Complex Molecules of Ethidium Bromide and Plasmid DNA in Solution by Atomic Force Microscopy

Tertiary structure changes in plasmid DNA, induced by ethidium bromide intercalation, have been observed in aqueous solutions by the use of an atomic force microscoe. A relaxed closed circular pBR322 molecule became a positively supercoiled complex on the drug binding. The supercoiling always result...

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Veröffentlicht in:	Chemical & pharmaceutical bulletin 2001, Vol.49(4), pp.413-417
Hauptverfasser:	UTSUNO, Kuniharu, TSUBOI, Masamichi, KATSUMATA, Shunji, IWAMOTO, Toschitake
Format:	Artikel
Sprache:	eng
Schlagworte:	atomic force microscopy Biological and medical sciences DNA - chemistry DNA - ultrastructure Electrophoresis, Polyacrylamide Gel Ethidium - chemistry ethidium bromide Fundamental and applied biological sciences. Psychology Intermolecular phenomena interwound form of DNA duplex Microscopy, Atomic Force Miscellaneous Molecular biophysics Nucleic Acid Conformation plasmid DNA Plasmids - chemistry Plasmids - ultrastructure plectonemic form of DNA duplex Solutions Spectrophotometry, Infrared
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Beschreibung
Zusammenfassung:	Tertiary structure changes in plasmid DNA, induced by ethidium bromide intercalation, have been observed in aqueous solutions by the use of an atomic force microscoe. A relaxed closed circular pBR322 molecule became a positively supercoiled complex on the drug binding. The supercoiling always resulted in an interwound (or a plectonemic) form, but never a solenoidal (or a toroidal) form. A quantitative analysis of the compactness of such supercoiled complexes has been carried out.
ISSN:	0009-2363 1347-5223
DOI:	10.1248/cpb.49.413