Species-dependent differences of the biochemical properties of diamine oxidase

1. 1. Diamine oxidase (DAO) from tissues of mice, rats and humans showed different properties with respect to stability and kinetic parameters. DAO-activities in homogenates of rat or human tissues, but not of mouse tissues, rapidly decreased upon storage at −20°C. 2. 2. The K m -value for putrescin...

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Veröffentlicht in:	International journal of biochemistry 1986, Vol.18 (9), p.835-839
Hauptverfasser:	Romijn, J.C., Verkoelen, C.F., Splinter, T.A.W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amine Oxidase (Copper-Containing) - metabolism Animals Carcinoma, Renal Cell - enzymology Cell Line Enzyme Stability Humans Kidney - enzymology Kidney Neoplasms - enzymology Kinetics Male Mice Mice, Inbred BALB C Rats Rats, Inbred Strains Species Specificity
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Zusammenfassung:	1. 1. Diamine oxidase (DAO) from tissues of mice, rats and humans showed different properties with respect to stability and kinetic parameters. DAO-activities in homogenates of rat or human tissues, but not of mouse tissues, rapidly decreased upon storage at −20°C. 2. 2. The K m -value for putrescine was 90 μM in mouse kidney or intestine. In rats different K m -values were observed before (272 μM) and after freezing (102 μM). 3. 3. A similar effect was observed with DAO in human kidney (321 and 39 μM, respectively). 4. 4. Treatment of rats with heparin resulted in a depletion of intestinal DAO and the concomitant appearance of DAO in blood. 5. 5. The enzyme remaining in the intestine showed the lower K m -value.
ISSN:	0020-711X
DOI:	10.1016/0020-711X(86)90061-3