The use of bacteriophage depolymerization in the structural investigation of the capsular polysaccharide from Klebsiella serotype K3

The structure of the repeating unit of the capsular polysaccharide from Klebsiella serotype K3 has been established from the results of n.m.r. ( 1H and 13C) spectroscopy and methylation analysis of (a) P1, the pyruvic acetal-bearing pentasaccharide obtained on depolymerization of the polysaccharide...

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Veröffentlicht in:	Carbohydrate research 1986-07, Vol.149 (2), p.411-423
Hauptverfasser:	Dutton, Guy G.S., Parolis, Haralambos, Joseleau, Jean-Paul, Marais, Marie-France
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriology Bacteriophages - enzymology Biological and medical sciences Carbohydrate Conformation Carbohydrate Sequence Chromatography, Gas Fundamental and applied biological sciences. Psychology Galactosidases - metabolism Klebsiella - immunology Magnetic Resonance Spectroscopy Methylation Microbiology Morphology, structure, chemical composition Oligosaccharides - analysis Optical Rotation Polysaccharides, Bacterial - isolation & purification Serotyping
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Zusammenfassung:	The structure of the repeating unit of the capsular polysaccharide from Klebsiella serotype K3 has been established from the results of n.m.r. ( 1H and 13C) spectroscopy and methylation analysis of (a) P1, the pyruvic acetal-bearing pentasaccharide obtained on depolymerization of the polysaccharide with a bacteriophage-borne endogalactosidase, (b) reduced deacetalated P1, and (c) the native polysaccharide. The data permit the assignment of the following structure to the repeating unit: ▪
ISSN:	0008-6215 1873-426X
DOI:	10.1016/S0008-6215(00)90061-2