Improving the in vitro antigen specific T cell proliferation assay: the use of interferon-alpha to elicit antigen specific stimulation and decrease bystander proliferation

The measurement of the proliferative response of primed T cells to an antigenic stimulus (lymphocyte transformation assay: LTT) is commonly used for determining T cell immune responsiveness. However, the ratio between the spontaneous and the antigen-triggered response (stimulation index) is frequent...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of immunological methods 2001-05, Vol.251 (1), p.63-71
Hauptverfasser:	von Baehr, Volker, Mayer, Wolfgang, Liebenthal, Christa, von Baehr, Rüdiger, Bieger, Wilfried, Volk, Hans-Dieter
Format:	Artikel
Sprache:	eng
Schlagworte:	a-Interferon Adult Antibiotics. Antiinfectious agents. Antiparasitic agents Antifungal agents Antigen-specificity Antigens - administration & dosage Antigens, Fungal - administration & dosage Antigens, Viral - administration & dosage Biological and medical sciences Candida albicans Candida albicans - immunology Encephalitis Viruses, Tick-Borne - immunology Female Fundamental and applied biological sciences. Psychology Fundamental immunology Humans In Vitro Techniques Interferon Interferon alpha-2 Interferon-alpha - administration & dosage Kinetics Lymphocyte Activation Lymphocyte transformation test Male Medical sciences Memory T cells Microbiology Middle Aged Molecular immunology Pharmacology. Drug treatments Recombinant Proteins T-Lymphocytes - immunology Techniques Tetanus Toxoid - administration & dosage
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The measurement of the proliferative response of primed T cells to an antigenic stimulus (lymphocyte transformation assay: LTT) is commonly used for determining T cell immune responsiveness. However, the ratio between the spontaneous and the antigen-triggered response (stimulation index) is frequently quite low (
ISSN:	0022-1759 1872-7905
DOI:	10.1016/S0022-1759(01)00297-6