Sulfated β-(1→4)-galacto-oligosaccharides and their effect on angiogenesis

Sulfated β-(1→4)-galacto-oligosaccharides were prepared from an arabino-galacto-rhamno-galacturonan from Lupinus polyphyllus Lindl. by successive partial hydrolysis and SO 3–pyridine sulfation in DMF. The resulting oligosaccharide polysulfates were analyzed by analytical GPC and the sulfate content...

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Veröffentlicht in:	Carbohydrate research 2001-02, Vol.330 (3), p.427-430
Hauptverfasser:	Käsbauer, Christian W, Paper, Dietrich H, Franz, Gerhard
Format:	Artikel
Sprache:	eng
Schlagworte:	Allantois - blood supply Angiogenesis inhibitor Angiogenesis Inhibitors - chemistry Angiogenesis Inhibitors - isolation & purification Angiogenesis Inhibitors - pharmacology Animals CAM-assay Carbohydrate Conformation Carbohydrate Sequence Chick Embryo Chorion - blood supply Fabaceae - chemistry FGF-2, Galactan Molecular Sequence Data Neovascularization, Physiologic - drug effects Oligosaccharides - chemistry Oligosaccharides - isolation & purification Oligosaccharides - pharmacology Plants, Medicinal Polysaccharides - chemistry Polysaccharides - isolation & purification Sulfated galacto-oligosaccharides
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Zusammenfassung:	Sulfated β-(1→4)-galacto-oligosaccharides were prepared from an arabino-galacto-rhamno-galacturonan from Lupinus polyphyllus Lindl. by successive partial hydrolysis and SO 3–pyridine sulfation in DMF. The resulting oligosaccharide polysulfates were analyzed by analytical GPC and the sulfate content was determined by ion chromatography. DP 5 and higher showed a pronounced antiangiogenic effect with scores of 0.9–1.2 for DP 7–9 using the CAM-assay. An interaction with the fibroblast growth factor FGF-2 was noticed for DP 4–12 depending on the degree of sulfation using the FGF-2-trypsin assay.
ISSN:	0008-6215 1873-426X
DOI:	10.1016/S0008-6215(00)00305-0