Induction of heme oxygenase 1 in liver of spontaneously diabetic rats

It has been suggested that diabetes induces an increase in oxidative stress; the increased expression of heme-oxygenase 1 (HO-1) in liver is believed to be a sensitive marker of the stress response. The aim of this study was to examine whether diabetes is able to induce HO-1 expression in liver. The...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Free radical research 2001-01, Vol.34 (2), p.189-191
Hauptverfasser:	Cosso, L., Maineri, E. Pesce, Traverso, N., Rosatto, N., Pronzato, M.A., Cottalasso, D., Marinari, U.M., Odetti, P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Diabetes Mellitus, Type 1 - enzymology Disease Models, Animal Enzyme Induction heme oxygenase Hyperglycemia - enzymology Liver - enzymology Male Oxidative Stress Rats Rats, Inbred BB Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - biosynthesis RT/PCR stress protein
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	It has been suggested that diabetes induces an increase in oxidative stress; the increased expression of heme-oxygenase 1 (HO-1) in liver is believed to be a sensitive marker of the stress response. The aim of this study was to examine whether diabetes is able to induce HO-1 expression in liver. The specific mRNA was amplified by RT/PCR and calibrated with amplified β-actin mRNA. The mRNA HO-1 levels in the liver of spontaneously diabetic rats were increased by 1.8 fold compared with non diabetics; this supports the hypothesis of weak but significant oxidative damage due to chronic hyperglycaemia. This work represents the first in vivo study exploring the semi-quantitative expression of HO-1 in the liver of spontaneously diabetic rats.
ISSN:	1071-5762 1029-2470
DOI:	10.1080/10715760100300171