A fiber-optic lactate sensor based on bacterial cytoplasmic membranes
A new type of fiber-optic biosensor based on bacterial cytoplasmic membranes (CPM) as the biological recognition element and an oxygen sensitive dye layer as the transducer is described for the detection of lactate. CPMs from bacteria with an induced lactate oxidase system are adsorbed onto a cellul...
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Veröffentlicht in: | Biosensors & bioelectronics 2001, Vol.16 (1), p.109-113 |
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creator | Ignatov, Sergei G. Ferguson, Jane A. Walt, David R. |
description | A new type of fiber-optic biosensor based on bacterial cytoplasmic membranes (CPM) as the biological recognition element and an oxygen sensitive dye layer as the transducer is described for the detection of lactate. CPMs from bacteria with an induced lactate oxidase system are adsorbed onto a cellulose disk. The disk is fixed mechanically over an oxygen sensitive siloxane layer on the distal end of an optical fiber. This system detects lactate with no interference from glucose, fructose or glutamic acid. |
doi_str_mv | 10.1016/S0956-5663(00)00144-5 |
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This system detects lactate with no interference from glucose, fructose or glutamic acid.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/S0956-5663(00)00144-5</identifier><identifier>PMID: 11261845</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Bacteria ; Biosensing Techniques ; Cell Membrane ; Cytoplasmic membrane ; Fiber Optic Technology ; Glucose ; Lactic Acid - analysis ; Optical Fibers</subject><ispartof>Biosensors & bioelectronics, 2001, Vol.16 (1), p.109-113</ispartof><rights>2001 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-f1f8c7eaee4fe11c9deb5bfbde91794f38b575321a0dc2d0040b7d5e8b0551ca3</citedby><cites>FETCH-LOGICAL-c392t-f1f8c7eaee4fe11c9deb5bfbde91794f38b575321a0dc2d0040b7d5e8b0551ca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0956-5663(00)00144-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,4023,27922,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11261845$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ignatov, Sergei G.</creatorcontrib><creatorcontrib>Ferguson, Jane A.</creatorcontrib><creatorcontrib>Walt, David R.</creatorcontrib><title>A fiber-optic lactate sensor based on bacterial cytoplasmic membranes</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>A new type of fiber-optic biosensor based on bacterial cytoplasmic membranes (CPM) as the biological recognition element and an oxygen sensitive dye layer as the transducer is described for the detection of lactate. CPMs from bacteria with an induced lactate oxidase system are adsorbed onto a cellulose disk. The disk is fixed mechanically over an oxygen sensitive siloxane layer on the distal end of an optical fiber. This system detects lactate with no interference from glucose, fructose or glutamic acid.</description><subject>Bacteria</subject><subject>Biosensing Techniques</subject><subject>Cell Membrane</subject><subject>Cytoplasmic membrane</subject><subject>Fiber Optic Technology</subject><subject>Glucose</subject><subject>Lactic Acid - analysis</subject><subject>Optical Fibers</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKxEAQRRtRnHH0E5SsRBfRqiSdTlYyDOMDBlyo66YfFWjJy-6MMH9v5oEuXVUtzq1bHMYuEe4QML9_g5LnMc_z9AbgFgCzLOZHbIqFSOMsSfkxm_4iE3YWwicACCzhlE0QkxyLjE_Zch5VTpOPu35wJqqVGdRAUaA2dD7SKpCNunZczEDeqToym6HraxWakW6o0V61FM7ZSaXqQBeHOWMfj8v3xXO8en16WcxXsUnLZIgrrAojSBFlFSGa0pLmutKWShRlVqWF5oKnCSqwJrEAGWhhORUaOEej0hm73t_tffe1pjDIxgVDdT0-0a2DFHlZcMz5v2CSi0ykAkeQ70HjuxA8VbL3rlF-IxHkVrTciZZbixJA7kTLbcHVoWCtG7J_qYPZEXjYAzT6-HbkZTCOWkPWeTKDtJ37p-IHVmKNtQ</recordid><startdate>2001</startdate><enddate>2001</enddate><creator>Ignatov, Sergei G.</creator><creator>Ferguson, Jane A.</creator><creator>Walt, David R.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>2001</creationdate><title>A fiber-optic lactate sensor based on bacterial cytoplasmic membranes</title><author>Ignatov, Sergei G. ; Ferguson, Jane A. ; Walt, David R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-f1f8c7eaee4fe11c9deb5bfbde91794f38b575321a0dc2d0040b7d5e8b0551ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Bacteria</topic><topic>Biosensing Techniques</topic><topic>Cell Membrane</topic><topic>Cytoplasmic membrane</topic><topic>Fiber Optic Technology</topic><topic>Glucose</topic><topic>Lactic Acid - analysis</topic><topic>Optical Fibers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ignatov, Sergei G.</creatorcontrib><creatorcontrib>Ferguson, Jane A.</creatorcontrib><creatorcontrib>Walt, David R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ignatov, Sergei G.</au><au>Ferguson, Jane A.</au><au>Walt, David R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A fiber-optic lactate sensor based on bacterial cytoplasmic membranes</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2001</date><risdate>2001</risdate><volume>16</volume><issue>1</issue><spage>109</spage><epage>113</epage><pages>109-113</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>A new type of fiber-optic biosensor based on bacterial cytoplasmic membranes (CPM) as the biological recognition element and an oxygen sensitive dye layer as the transducer is described for the detection of lactate. 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source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
subjects | Bacteria Biosensing Techniques Cell Membrane Cytoplasmic membrane Fiber Optic Technology Glucose Lactic Acid - analysis Optical Fibers |
title | A fiber-optic lactate sensor based on bacterial cytoplasmic membranes |
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