A fiber-optic lactate sensor based on bacterial cytoplasmic membranes

A fiber-optic lactate sensor based on bacterial cytoplasmic membranes

A new type of fiber-optic biosensor based on bacterial cytoplasmic membranes (CPM) as the biological recognition element and an oxygen sensitive dye layer as the transducer is described for the detection of lactate. CPMs from bacteria with an induced lactate oxidase system are adsorbed onto a cellul...

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Veröffentlicht in:Biosensors & bioelectronics 2001, Vol.16 (1), p.109-113
Hauptverfasser: Ignatov, Sergei G., Ferguson, Jane A., Walt, David R.
Format: Artikel
Sprache:eng
Schlagworte:
Bacteria
Biosensing Techniques
Cell Membrane
Cytoplasmic membrane
Fiber Optic Technology
Glucose
Lactic Acid - analysis
Optical Fibers
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Zusammenfassung:A new type of fiber-optic biosensor based on bacterial cytoplasmic membranes (CPM) as the biological recognition element and an oxygen sensitive dye layer as the transducer is described for the detection of lactate. CPMs from bacteria with an induced lactate oxidase system are adsorbed onto a cellulose disk. The disk is fixed mechanically over an oxygen sensitive siloxane layer on the distal end of an optical fiber. This system detects lactate with no interference from glucose, fructose or glutamic acid.
ISSN:0956-5663
1873-4235
DOI:10.1016/S0956-5663(00)00144-5