Validation of a polymerase chain reaction assay for monitoring the therapeutic efficacy of diminazene aceturate in trypanosome-infected sheep

The diagnostic performance of a polymerase chain reaction assay (PCR) for monitoring the effectiveness of aceturate diminazene treatment was compared with those of an antibody-detection ELISA test and the buffy-coat technique using sheep experimentally infected with either savannah-type or forest-ty...

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Veröffentlicht in:	Veterinary parasitology 2001-03, Vol.96 (2), p.101-113
Hauptverfasser:	Bengaly, Z, Kasbari, M, Desquesnes, M, Sidibé, I
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Protozoan - analysis Control methods-Protoza Diminazene - analogs & derivatives Diminazene - therapeutic use diminazene aceturate Enzyme-Linked Immunosorbent Assay - veterinary Experimental infection PCR Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary Sheep Sheep Diseases - drug therapy Sheep-Protozoa Trypanocidal Agents - therapeutic use Trypanosoma congolense Trypanosoma spp Trypanosoma vivax Trypanosomiasis - drug therapy Trypanosomiasis - veterinary Validation
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Beschreibung
Zusammenfassung:	The diagnostic performance of a polymerase chain reaction assay (PCR) for monitoring the effectiveness of aceturate diminazene treatment was compared with those of an antibody-detection ELISA test and the buffy-coat technique using sheep experimentally infected with either savannah-type or forest-type Trypanosoma congolense or T. vivax. Within the period of infection, the PCR using specific savannah-type T. congolense primers showed a significant higher diagnostic sensitivity ( p
ISSN:	0304-4017 1873-2550
DOI:	10.1016/S0304-4017(00)00426-X