Tissue-dependent enhancement of transgene expression by introns of replacement histone H3 genes of Arabidopsis
Intron-bearing replacement histone H3 genes in Arabidopsis and other plants are highly and constitutively expressed. We demonstrate that the introns located within the 5'-untranslated regions (5'-UTR) of the two Arabidopsis replacement H3 genes will abolish the cell cycle dependence of an...
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| Veröffentlicht in: | Plant molecular biology 2001-01, Vol.45 (1), p.17-30 |
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| creator | Chaubet-Gigot, N Kapros, T Flenet, M Kahn, K Gigot, C Waterborg, J H |
| description | Intron-bearing replacement histone H3 genes in Arabidopsis and other plants are highly and constitutively expressed. We demonstrate that the introns located within the 5'-untranslated regions (5'-UTR) of the two Arabidopsis replacement H3 genes will abolish the cell cycle dependence of an endogenous histone H4 promoter. We demonstrate that these introns, functionally combined with their endogenous promoters, could produce the high and constitutive expression of the replacement H3 genes observed in planta. They strongly increase gene expression whatever the promoter, from the strong 35S CaMV promoter to complete and resected promoters of cell cycle-dependent and replacement histone genes. Quantitative analysis of the extent of reporter gene enhancement in different parts of developing transgenic plantlets, ranging from 2-fold to 70-fold, supports the notion that trans-acting factors are responsible for this effect. Such factors appear most abundant in roots. |
| doi_str_mv | 10.1023/A:1006487023926 |
| format | Article |
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We demonstrate that the introns located within the 5'-untranslated regions (5'-UTR) of the two Arabidopsis replacement H3 genes will abolish the cell cycle dependence of an endogenous histone H4 promoter. We demonstrate that these introns, functionally combined with their endogenous promoters, could produce the high and constitutive expression of the replacement H3 genes observed in planta. They strongly increase gene expression whatever the promoter, from the strong 35S CaMV promoter to complete and resected promoters of cell cycle-dependent and replacement histone genes. Quantitative analysis of the extent of reporter gene enhancement in different parts of developing transgenic plantlets, ranging from 2-fold to 70-fold, supports the notion that trans-acting factors are responsible for this effect. Such factors appear most abundant in roots.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1023/A:1006487023926</identifier><identifier>PMID: 11247603</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Arabidopsis ; Arabidopsis - genetics ; Caulimovirus - genetics ; Cell cycle ; DNA, Plant - genetics ; Gene expression ; Gene Expression Regulation, Plant ; Glucuronidase - genetics ; Glucuronidase - metabolism ; Histones - genetics ; Introns - genetics ; Plant Roots - genetics ; Plant Roots - metabolism ; Plants, Genetically Modified ; Promoter Regions, Genetic - genetics ; Proteins ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; Tissue Distribution ; Transformation, Genetic ; transgenes ; Transgenes - genetics</subject><ispartof>Plant molecular biology, 2001-01, Vol.45 (1), p.17-30</ispartof><rights>Kluwer Academic Publishers 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11247603$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chaubet-Gigot, N</creatorcontrib><creatorcontrib>Kapros, T</creatorcontrib><creatorcontrib>Flenet, M</creatorcontrib><creatorcontrib>Kahn, K</creatorcontrib><creatorcontrib>Gigot, C</creatorcontrib><creatorcontrib>Waterborg, J H</creatorcontrib><title>Tissue-dependent enhancement of transgene expression by introns of replacement histone H3 genes of Arabidopsis</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>Intron-bearing replacement histone H3 genes in Arabidopsis and other plants are highly and constitutively expressed. We demonstrate that the introns located within the 5'-untranslated regions (5'-UTR) of the two Arabidopsis replacement H3 genes will abolish the cell cycle dependence of an endogenous histone H4 promoter. We demonstrate that these introns, functionally combined with their endogenous promoters, could produce the high and constitutive expression of the replacement H3 genes observed in planta. They strongly increase gene expression whatever the promoter, from the strong 35S CaMV promoter to complete and resected promoters of cell cycle-dependent and replacement histone genes. Quantitative analysis of the extent of reporter gene enhancement in different parts of developing transgenic plantlets, ranging from 2-fold to 70-fold, supports the notion that trans-acting factors are responsible for this effect. Such factors appear most abundant in roots.</description><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Caulimovirus - genetics</subject><subject>Cell cycle</subject><subject>DNA, Plant - genetics</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Glucuronidase - genetics</subject><subject>Glucuronidase - metabolism</subject><subject>Histones - genetics</subject><subject>Introns - genetics</subject><subject>Plant Roots - genetics</subject><subject>Plant Roots - metabolism</subject><subject>Plants, Genetically Modified</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Proteins</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Tissue Distribution</subject><subject>Transformation, Genetic</subject><subject>transgenes</subject><subject>Transgenes - genetics</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkD1PwzAQhi0EoqUws6GIgS1gx99sVcWXVImlzJGTnKmr1Al2ItF_jwtlYeGWu9M97-nuReiS4FuCC3o3vycYC6ZkanQhjtCUcElzjgt1jKaYCJkzRooJOotxg3GCqThFE0IKJgWmU-RXLsYR8gZ68A34IQO_Nr6G7b7ubDYE4-M7eMjgsw8Qo-t8Vu0y54fQ-bhHAvStOSjWLg5dgp9pthd9z-fBVK7p-ujiOTqxpo1wccgz9Pb4sFo858vXp5fFfJn3RMshFxXR1NJCCKVsRSnDVteGF8QaKZUQghOSzudMU6C6ZspoUllpleaCSa3pDN387O1D9zFCHMqtizW0rfHQjbGUQnMtGP4XJDIFTZ7O0PUfcNONwacnSimxkJwrkaCrAzRWW2jKPritCbvy12_6BTMngRg</recordid><startdate>200101</startdate><enddate>200101</enddate><creator>Chaubet-Gigot, N</creator><creator>Kapros, T</creator><creator>Flenet, M</creator><creator>Kahn, K</creator><creator>Gigot, C</creator><creator>Waterborg, J H</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200101</creationdate><title>Tissue-dependent enhancement of transgene expression by introns of replacement histone H3 genes of Arabidopsis</title><author>Chaubet-Gigot, N ; 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We demonstrate that the introns located within the 5'-untranslated regions (5'-UTR) of the two Arabidopsis replacement H3 genes will abolish the cell cycle dependence of an endogenous histone H4 promoter. We demonstrate that these introns, functionally combined with their endogenous promoters, could produce the high and constitutive expression of the replacement H3 genes observed in planta. They strongly increase gene expression whatever the promoter, from the strong 35S CaMV promoter to complete and resected promoters of cell cycle-dependent and replacement histone genes. Quantitative analysis of the extent of reporter gene enhancement in different parts of developing transgenic plantlets, ranging from 2-fold to 70-fold, supports the notion that trans-acting factors are responsible for this effect. 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| subjects | Arabidopsis Arabidopsis - genetics Caulimovirus - genetics Cell cycle DNA, Plant - genetics Gene expression Gene Expression Regulation, Plant Glucuronidase - genetics Glucuronidase - metabolism Histones - genetics Introns - genetics Plant Roots - genetics Plant Roots - metabolism Plants, Genetically Modified Promoter Regions, Genetic - genetics Proteins Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Tissue Distribution Transformation, Genetic transgenes Transgenes - genetics |
| title | Tissue-dependent enhancement of transgene expression by introns of replacement histone H3 genes of Arabidopsis |
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