Aujeszky's disease: Blocking ELISA for the detection of serum antibodies
A blocking ELISA for the detection of serum antibodies to Aujeszky's disease virus has been developed. The test was designed in particular with a view to examining large numbers of blood samples. It has been found to be sensitive, specific and precise. In comparison with the serum neutralizatio...
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| Veröffentlicht in: | Journal of virological methods 1986-05, Vol.13 (2), p.171-181 |
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| container_title | Journal of virological methods |
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| creator | Sørensen, K.J. Lei, J.C. |
| description | A blocking ELISA for the detection of serum antibodies to Aujeszky's disease virus has been developed. The test was designed in particular with a view to examining large numbers of blood samples. It has been found to be sensitive, specific and precise. In comparison with the serum neutralization test it was superior in detecting low levels of antibodies in pig sera. The blocking ELISA gave higher titre values in serum samples taken early after experimental infection of pigs with Aujeszky's disease virus, than did the serum neutralization test. |
| doi_str_mv | 10.1016/0166-0934(86)90085-6 |
| format | Article |
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The blocking ELISA gave higher titre values in serum samples taken early after experimental infection of pigs with Aujeszky's disease virus, than did the serum neutralization test.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/0166-0934(86)90085-6</identifier><identifier>PMID: 3016016</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antibodies, Viral - analysis ; Aujeszky's disease ; Cells, Cultured ; ELISA ; Enzyme-Linked Immunosorbent Assay ; Herpesvirus 1, Suid - immunology ; Neutralization Tests ; Pseudorabies - diagnosis ; Pseudorabies - immunology ; pseudorabies virus ; serum antibodies ; Swine ; Swine Diseases - diagnosis ; Swine Diseases - immunology</subject><ispartof>Journal of virological methods, 1986-05, Vol.13 (2), p.171-181</ispartof><rights>1986</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-85a63710e07558b19af36b8024e81836518dfc12f083f0a6374ea956ff29a9033</citedby><cites>FETCH-LOGICAL-c388t-85a63710e07558b19af36b8024e81836518dfc12f083f0a6374ea956ff29a9033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0166-0934(86)90085-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3016016$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sørensen, K.J.</creatorcontrib><creatorcontrib>Lei, J.C.</creatorcontrib><title>Aujeszky's disease: Blocking ELISA for the detection of serum antibodies</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>A blocking ELISA for the detection of serum antibodies to Aujeszky's disease virus has been developed. The test was designed in particular with a view to examining large numbers of blood samples. It has been found to be sensitive, specific and precise. In comparison with the serum neutralization test it was superior in detecting low levels of antibodies in pig sera. The blocking ELISA gave higher titre values in serum samples taken early after experimental infection of pigs with Aujeszky's disease virus, than did the serum neutralization test.</description><subject>Animals</subject><subject>Antibodies, Viral - analysis</subject><subject>Aujeszky's disease</subject><subject>Cells, Cultured</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Herpesvirus 1, Suid - immunology</subject><subject>Neutralization Tests</subject><subject>Pseudorabies - diagnosis</subject><subject>Pseudorabies - immunology</subject><subject>pseudorabies virus</subject><subject>serum antibodies</subject><subject>Swine</subject><subject>Swine Diseases - diagnosis</subject><subject>Swine Diseases - immunology</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LAzEQhoMotX78A4Wc_DisTja72cSDUKV-QMGDeg7p7kSj7UaTXUF_vaktHhUyBOZ9ZgYeQvYYnDBg4jSVyEDx4kiKYwUgy0yskSGTlUptWayT4S-ySbZifAGAsuJ8QAY8JekNyc2of8H49fp5GGnjIpqIZ_Ri5utX1z7R8eT2fkStD7R7Rtpgh3XnfEu9pRFDP6em7dzUNw7jDtmwZhZxd_Vvk8er8cPlTTa5u769HE2ymkvZZbI0glcMEKqylFOmjOViKiEvUDLJRclkY2uWW5DcwoIt0KhSWJsro4DzbXKw3PsW_HuPsdNzF2uczUyLvo-6Eqosqvx_kBUFKMghgcUSrIOPMaDVb8HNTfjUDPTCtF5o1AuNWgr9Y1qLNLa_2t9P59j8Dq3Upvx8mWOy8eEw6Fg7bGtsXEgadePd3we-AQsYing</recordid><startdate>19860501</startdate><enddate>19860501</enddate><creator>Sørensen, K.J.</creator><creator>Lei, J.C.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19860501</creationdate><title>Aujeszky's disease: Blocking ELISA for the detection of serum antibodies</title><author>Sørensen, K.J. ; Lei, J.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-85a63710e07558b19af36b8024e81836518dfc12f083f0a6374ea956ff29a9033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Antibodies, Viral - analysis</topic><topic>Aujeszky's disease</topic><topic>Cells, Cultured</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Herpesvirus 1, Suid - immunology</topic><topic>Neutralization Tests</topic><topic>Pseudorabies - diagnosis</topic><topic>Pseudorabies - immunology</topic><topic>pseudorabies virus</topic><topic>serum antibodies</topic><topic>Swine</topic><topic>Swine Diseases - diagnosis</topic><topic>Swine Diseases - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sørensen, K.J.</creatorcontrib><creatorcontrib>Lei, J.C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sørensen, K.J.</au><au>Lei, J.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aujeszky's disease: Blocking ELISA for the detection of serum antibodies</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>1986-05-01</date><risdate>1986</risdate><volume>13</volume><issue>2</issue><spage>171</spage><epage>181</epage><pages>171-181</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><abstract>A blocking ELISA for the detection of serum antibodies to Aujeszky's disease virus has been developed. 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| identifier | ISSN: 0166-0934 |
| ispartof | Journal of virological methods, 1986-05, Vol.13 (2), p.171-181 |
| issn | 0166-0934 1879-0984 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Antibodies, Viral - analysis Aujeszky's disease Cells, Cultured ELISA Enzyme-Linked Immunosorbent Assay Herpesvirus 1, Suid - immunology Neutralization Tests Pseudorabies - diagnosis Pseudorabies - immunology pseudorabies virus serum antibodies Swine Swine Diseases - diagnosis Swine Diseases - immunology |
| title | Aujeszky's disease: Blocking ELISA for the detection of serum antibodies |
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