Aujeszky's disease: Blocking ELISA for the detection of serum antibodies

A blocking ELISA for the detection of serum antibodies to Aujeszky's disease virus has been developed. The test was designed in particular with a view to examining large numbers of blood samples. It has been found to be sensitive, specific and precise. In comparison with the serum neutralizatio...

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Veröffentlicht in:	Journal of virological methods 1986-05, Vol.13 (2), p.171-181
Hauptverfasser:	Sørensen, K.J., Lei, J.C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Viral - analysis Aujeszky's disease Cells, Cultured ELISA Enzyme-Linked Immunosorbent Assay Herpesvirus 1, Suid - immunology Neutralization Tests Pseudorabies - diagnosis Pseudorabies - immunology pseudorabies virus serum antibodies Swine Swine Diseases - diagnosis Swine Diseases - immunology
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Beschreibung
Zusammenfassung:	A blocking ELISA for the detection of serum antibodies to Aujeszky's disease virus has been developed. The test was designed in particular with a view to examining large numbers of blood samples. It has been found to be sensitive, specific and precise. In comparison with the serum neutralization test it was superior in detecting low levels of antibodies in pig sera. The blocking ELISA gave higher titre values in serum samples taken early after experimental infection of pigs with Aujeszky's disease virus, than did the serum neutralization test.
ISSN:	0166-0934 1879-0984
DOI:	10.1016/0166-0934(86)90085-6