A highly sensitive assay method for human placental alkaline phosphatase involving a monoclonal antibody bound to a paper disk

A monoclonal antibody which is specific for human placental alkaline phosphatase and does not cross-react at all with intestinal alkaline phosphatase was prepared, and a procedure for the determination of placental alkaline phosphatase activity in serum was developed involving this tnonoclonal antib...

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Veröffentlicht in:Analytical biochemistry 1986-05, Vol.154 (2), p.624-631
Hauptverfasser: Hirano, Kazuyuki, Iiizumi, Yuichi, Hayashi, Yuji, Tanaka, Tsuyoshi, Sugiura, Mamoru, Hayashi, Kyozo, Lu, Zhen-Da, Iino, Shiro
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Sprache:eng
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Zusammenfassung:A monoclonal antibody which is specific for human placental alkaline phosphatase and does not cross-react at all with intestinal alkaline phosphatase was prepared, and a procedure for the determination of placental alkaline phosphatase activity in serum was developed involving this tnonoclonal antibody bound to a paper disk. The minimum amount of placental alkaline phosphatase detectable by this method is 0.0025 King-Armstrong unit. Good correlation with the heat-treatment method was obtained. Therefore this proposed method can be used as a routine clinical test for the determination of serum placental alkaline phosphatase.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(86)90038-2