A highly sensitive assay method for human placental alkaline phosphatase involving a monoclonal antibody bound to a paper disk
A monoclonal antibody which is specific for human placental alkaline phosphatase and does not cross-react at all with intestinal alkaline phosphatase was prepared, and a procedure for the determination of placental alkaline phosphatase activity in serum was developed involving this tnonoclonal antib...
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Veröffentlicht in: | Analytical biochemistry 1986-05, Vol.154 (2), p.624-631 |
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Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | A monoclonal antibody which is specific for human placental alkaline phosphatase and does not cross-react at all with intestinal alkaline phosphatase was prepared, and a procedure for the determination of placental alkaline phosphatase activity in serum was developed involving this tnonoclonal antibody bound to a paper disk. The minimum amount of placental alkaline phosphatase detectable by this method is 0.0025 King-Armstrong unit. Good correlation with the heat-treatment method was obtained. Therefore this proposed method can be used as a routine clinical test for the determination of serum placental alkaline phosphatase. |
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ISSN: | 0003-2697 1096-0309 |
DOI: | 10.1016/0003-2697(86)90038-2 |