Determination of the antigenic variability of pseudorabies virus field isolates with monoclonal antibodies

Five stable hybridoma cell lines secreting monoclonal antibodies (MAbs) were obtained by fusing spleen cells of pseudorabies virus (strain BUK) immunized BALB/c mice with mouse myeloma cell line SP2/0. Plate-trapped and antibody-trapped antigen ELISAs were done to compare the interaction of MAbs wit...

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Veröffentlicht in:	Acta veterinaria Hungarica (Budapest. 1983) 1994, Vol.42 (2-3), p.343-346
Hauptverfasser:	Matisová, J, Lesko, J, Gallo, J, Subr, Z, Sabo, A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - immunology Antigenic Variation - immunology Cell Line Cells, Cultured Chick Embryo Enzyme-Linked Immunosorbent Assay - veterinary Herpesvirus 1, Suid - immunology Herpesvirus 1, Suid - isolation & purification Hybridomas - immunology Kidney - virology Mice Mice, Inbred BALB C Swine - virology Virus Cultivation
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Beschreibung
Zusammenfassung:	Five stable hybridoma cell lines secreting monoclonal antibodies (MAbs) were obtained by fusing spleen cells of pseudorabies virus (strain BUK) immunized BALB/c mice with mouse myeloma cell line SP2/0. Plate-trapped and antibody-trapped antigen ELISAs were done to compare the interaction of MAbs with TOP and "K" strains and 7 Slovak field isolates. Only 2 Slovak isolates reacted with all the MAbs, 3 MAbs reacted with TOP and "K" strains. One Slovak isolate gave no reaction with any MAbs.
ISSN:	0236-6290