Enzyme electrode for the measurement of lactate in undiluted blood
An enzyme electrode is described based upon the enzyme lactate oxidase (EC 1.1.3.2) coupled to an H 2O 2 sensor. Use of an organosilane-treated microporous membrane over the enzyme layer, allows responses linear to 18 mmol/l l-lactate with response times of 1–3 min, whilst giving independence from s...
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| Veröffentlicht in: | Clinica chimica acta 1986-06, Vol.157 (2), p.191-198 |
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| container_end_page | 198 |
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| container_issue | 2 |
| container_start_page | 191 |
| container_title | Clinica chimica acta |
| container_volume | 157 |
| creator | Mullen, W.H. Churchouse, S.J. Keedy, F.H. Vadgama, P.M. |
| description | An enzyme electrode is described based upon the enzyme lactate oxidase (EC 1.1.3.2) coupled to an H
2O
2 sensor. Use of an organosilane-treated microporous membrane over the enzyme layer, allows responses linear to 18 mmol/l
l-lactate with response times of 1–3 min, whilst giving independence from stirring. The method is suitable for the bedside analysis of a single drop of undiluted whole blood. Correlation with a routine method gave
y = 0.96
x + 0.11,
r = 0.996,
n = 46. |
| doi_str_mv | 10.1016/0009-8981(86)90225-1 |
| format | Article |
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2O
2 sensor. Use of an organosilane-treated microporous membrane over the enzyme layer, allows responses linear to 18 mmol/l
l-lactate with response times of 1–3 min, whilst giving independence from stirring. The method is suitable for the bedside analysis of a single drop of undiluted whole blood. Correlation with a routine method gave
y = 0.96
x + 0.11,
r = 0.996,
n = 46.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/0009-8981(86)90225-1</identifier><identifier>PMID: 3719998</identifier><identifier>CODEN: CCATAR</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Cellulose - analogs & derivatives ; Diffusion ; Electrodes ; Enzyme electrode ; Fundamental and applied biological sciences. Psychology ; Humans ; Hydrogen-Ion Concentration ; Intermediary metabolites. Miscellaneous ; Lactate analysis ; Lactate oxidase ; Lactates - blood ; Lactic Acid ; Mixed Function Oxygenases ; Other biological molecules ; Silanes ; Whole blood measurement</subject><ispartof>Clinica chimica acta, 1986-06, Vol.157 (2), p.191-198</ispartof><rights>1986</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-f8d7fa3c8d3eab22c5a4af03d2aedfeb4c15ffac49ab4cbcfa4e996bc3aead323</citedby><cites>FETCH-LOGICAL-c386t-f8d7fa3c8d3eab22c5a4af03d2aedfeb4c15ffac49ab4cbcfa4e996bc3aead323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0009898186902251$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8790226$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3719998$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mullen, W.H.</creatorcontrib><creatorcontrib>Churchouse, S.J.</creatorcontrib><creatorcontrib>Keedy, F.H.</creatorcontrib><creatorcontrib>Vadgama, P.M.</creatorcontrib><title>Enzyme electrode for the measurement of lactate in undiluted blood</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>An enzyme electrode is described based upon the enzyme lactate oxidase (EC 1.1.3.2) coupled to an H
2O
2 sensor. Use of an organosilane-treated microporous membrane over the enzyme layer, allows responses linear to 18 mmol/l
l-lactate with response times of 1–3 min, whilst giving independence from stirring. The method is suitable for the bedside analysis of a single drop of undiluted whole blood. Correlation with a routine method gave
y = 0.96
x + 0.11,
r = 0.996,
n = 46.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Cellulose - analogs & derivatives</subject><subject>Diffusion</subject><subject>Electrodes</subject><subject>Enzyme electrode</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Intermediary metabolites. Miscellaneous</subject><subject>Lactate analysis</subject><subject>Lactate oxidase</subject><subject>Lactates - blood</subject><subject>Lactic Acid</subject><subject>Mixed Function Oxygenases</subject><subject>Other biological molecules</subject><subject>Silanes</subject><subject>Whole blood measurement</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMoun78A4UcRPRQTZpumlwEFb9A8KLnME0mGGkbTVpBf71dd9mjp5lhnnkZHkIOOTvnjMsLxpgulFb8VMkzzcpyXvANMuOqFoWodLlJZmtkh-zm_D6NFZN8m2yLmmut1Yxc3_Y_3x1SbNEOKTqkPiY6vCHtEPKYsMN-oNHTFuwAA9LQ07F3oR0HdLRpY3T7ZMtDm_FgVffI693ty81D8fR8_3hz9VRYoeRQeOVqD8IqJxCasrRzqMAz4UpA57GpLJ97D7bSMPWN9VCh1rKxAhCcKMUeOVnmfqT4OWIeTBeyxbaFHuOYTS2VlhWbT2C1BG2KOSf05iOFDtK34cws1JmFF7PwYpQ0f-oMn86OVvlj06FbH61cTfvj1R6yhdYn6G3Ia0zViyA5YZdLDCcXXwGTyTZgb9GFNDk2Lob___gFI7CMZg</recordid><startdate>19860615</startdate><enddate>19860615</enddate><creator>Mullen, W.H.</creator><creator>Churchouse, S.J.</creator><creator>Keedy, F.H.</creator><creator>Vadgama, P.M.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19860615</creationdate><title>Enzyme electrode for the measurement of lactate in undiluted blood</title><author>Mullen, W.H. ; Churchouse, S.J. ; Keedy, F.H. ; Vadgama, P.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-f8d7fa3c8d3eab22c5a4af03d2aedfeb4c15ffac49ab4cbcfa4e996bc3aead323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Cellulose - analogs & derivatives</topic><topic>Diffusion</topic><topic>Electrodes</topic><topic>Enzyme electrode</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Intermediary metabolites. Miscellaneous</topic><topic>Lactate analysis</topic><topic>Lactate oxidase</topic><topic>Lactates - blood</topic><topic>Lactic Acid</topic><topic>Mixed Function Oxygenases</topic><topic>Other biological molecules</topic><topic>Silanes</topic><topic>Whole blood measurement</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mullen, W.H.</creatorcontrib><creatorcontrib>Churchouse, S.J.</creatorcontrib><creatorcontrib>Keedy, F.H.</creatorcontrib><creatorcontrib>Vadgama, P.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mullen, W.H.</au><au>Churchouse, S.J.</au><au>Keedy, F.H.</au><au>Vadgama, P.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzyme electrode for the measurement of lactate in undiluted blood</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1986-06-15</date><risdate>1986</risdate><volume>157</volume><issue>2</issue><spage>191</spage><epage>198</epage><pages>191-198</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><coden>CCATAR</coden><abstract>An enzyme electrode is described based upon the enzyme lactate oxidase (EC 1.1.3.2) coupled to an H
2O
2 sensor. Use of an organosilane-treated microporous membrane over the enzyme layer, allows responses linear to 18 mmol/l
l-lactate with response times of 1–3 min, whilst giving independence from stirring. The method is suitable for the bedside analysis of a single drop of undiluted whole blood. Correlation with a routine method gave
y = 0.96
x + 0.11,
r = 0.996,
n = 46.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>3719998</pmid><doi>10.1016/0009-8981(86)90225-1</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-8981 |
| ispartof | Clinica chimica acta, 1986-06, Vol.157 (2), p.191-198 |
| issn | 0009-8981 1873-3492 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76896405 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Analytical, structural and metabolic biochemistry Biological and medical sciences Cellulose - analogs & derivatives Diffusion Electrodes Enzyme electrode Fundamental and applied biological sciences. Psychology Humans Hydrogen-Ion Concentration Intermediary metabolites. Miscellaneous Lactate analysis Lactate oxidase Lactates - blood Lactic Acid Mixed Function Oxygenases Other biological molecules Silanes Whole blood measurement |
| title | Enzyme electrode for the measurement of lactate in undiluted blood |
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