Enzyme electrode for the measurement of lactate in undiluted blood

An enzyme electrode is described based upon the enzyme lactate oxidase (EC 1.1.3.2) coupled to an H 2O 2 sensor. Use of an organosilane-treated microporous membrane over the enzyme layer, allows responses linear to 18 mmol/l l-lactate with response times of 1–3 min, whilst giving independence from s...

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Veröffentlicht in:	Clinica chimica acta 1986-06, Vol.157 (2), p.191-198
Hauptverfasser:	Mullen, W.H., Churchouse, S.J., Keedy, F.H., Vadgama, P.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Cellulose - analogs & derivatives Diffusion Electrodes Enzyme electrode Fundamental and applied biological sciences. Psychology Humans Hydrogen-Ion Concentration Intermediary metabolites. Miscellaneous Lactate analysis Lactate oxidase Lactates - blood Lactic Acid Mixed Function Oxygenases Other biological molecules Silanes Whole blood measurement
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Beschreibung
Zusammenfassung:	An enzyme electrode is described based upon the enzyme lactate oxidase (EC 1.1.3.2) coupled to an H 2O 2 sensor. Use of an organosilane-treated microporous membrane over the enzyme layer, allows responses linear to 18 mmol/l l-lactate with response times of 1–3 min, whilst giving independence from stirring. The method is suitable for the bedside analysis of a single drop of undiluted whole blood. Correlation with a routine method gave y = 0.96 x + 0.11, r = 0.996, n = 46.
ISSN:	0009-8981 1873-3492
DOI:	10.1016/0009-8981(86)90225-1