Cell-free synthesis of δ-(L-α-aminoadipyl)-L-cysteine, the first intermediate of penicillin and cephalosporin biosynthesis

Cell-free synthesis of δ-(L-α-aminoadipyl)-L-cysteine, the first intermediate of penicillin and cephalosporin biosynthesis

δ-(L-α-aminoadipyl)-L-cysteine synthetase (LL-AC synthetase) activity has been found in extracts of Cephalosporium acremonium C-10. The enzyme extract carries out a linear synthesis of LL-AC from its constituent amino acids for at least 6 hours. The reaction is dependent on active enzyme, time, L-α-...

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Veröffentlicht in:Biochemical and biophysical research communications 1986-05, Vol.137 (1), p.528-535
Hauptverfasser: Banko, Gerald, Wolfe, Saul, Demain, Arnold L.
Format: Artikel
Sprache:eng
Schlagworte:
Acremonium - metabolism
Adenosine Triphosphate - metabolism
Applied sciences
Cell-Free System
Cephalosporins - biosynthesis
Chromatography, High Pressure Liquid
Dipeptides - biosynthesis
Exact sciences and technology
Kinetics
Magnesium - metabolism
Manganese - metabolism
Other techniques and industries
Penicillins - biosynthesis
Temperature
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Zusammenfassung:δ-(L-α-aminoadipyl)-L-cysteine synthetase (LL-AC synthetase) activity has been found in extracts of Cephalosporium acremonium C-10. The enzyme extract carries out a linear synthesis of LL-AC from its constituent amino acids for at least 6 hours. The reaction is dependent on active enzyme, time, L-α-aminoadipate, L-cysteine, ATP and Mn 2+ or Mg 2+. The activity is stabilized by glycerol.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(86)91242-8