Flow cytofluorimetry of fluorescein fluorescence polarization to assay lymphocyte activation

Flow cytofluorimetry of fluorescein fluorescence polarization to assay lymphocyte activation

Change in fluorescence polarization of intracellular fluorescein measured with a specially adapted flow cytometer reliably reflected subtle biophysical changes in cells, such as those accompanying increased temperature or osmolality of the suspending medium. This system was developed to monitor chan...

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Veröffentlicht in:Biochemical and biophysical research communications 1986-05, Vol.136 (3), p.1021-1029
Hauptverfasser: Dimitropoulos, K., Rolland, J.M., Nairn, R.C.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Applied sciences
Exact sciences and technology
Flow Cytometry
Fluoresceins
Fluorescence Polarization
Lymphocyte Activation
Mice
Mice, Inbred BALB C
Osmolar Concentration
Other techniques and industries
Phytohemagglutinins - pharmacology
Staining and Labeling
Temperature
Time Factors
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Beschreibung
Zusammenfassung:Change in fluorescence polarization of intracellular fluorescein measured with a specially adapted flow cytometer reliably reflected subtle biophysical changes in cells, such as those accompanying increased temperature or osmolality of the suspending medium. This system was developed to monitor changes in lymphocytes one hour after stimulation with the mitogen phytohaemagglutinin, and provided a sensitive and rapid assay of lymphocyte activation.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(86)90435-3