Histone messenger RNAs of the mouse testis

A 6-12S RNA fraction has been isolated following sucrose gradient fractionation of mouse testis RNA, and further resolved into poly A+ and poly A- RNA fractions by oligo-(dt)-cellulose chromatography. Polyacrylamide gel electrophoresis of products formed in a reticulocyte lysate-dependent cell-free...

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Veröffentlicht in:	Biochemical and biophysical research communications 1986-05, Vol.136 (3), p.1116-1123
Hauptverfasser:	Faulkner, Robert D., Whisenant, Ethan C., Bhatnagar, Yogendra M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Applied sciences Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Exact sciences and technology histones Histones - genetics Male Mice Molecular Weight mRNA Other techniques and industries Poly A - analysis Protein Biosynthesis RNA - analysis RNA, Messenger - analysis testes Testis - analysis translation
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Zusammenfassung:	A 6-12S RNA fraction has been isolated following sucrose gradient fractionation of mouse testis RNA, and further resolved into poly A+ and poly A- RNA fractions by oligo-(dt)-cellulose chromatography. Polyacrylamide gel electrophoresis of products formed in a reticulocyte lysate-dependent cell-free translation system has enabled identification of histone variants, Hlt, H2S, H2A-X, an H4-like protein and a low M r protein (presumably TP and/or protamine). Cell-free synthesis of a number of these histone variants appears to be directed by poly A+ mRNAs.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/0006-291X(86)90449-3