In vivo observation of the human tear film by tandem scanning confocal microscopy

In vivo observation of the human tear film by tandem scanning confocal microscopy

A new method for observing normal and pathologic states of the human tear film using tandem scanning confocal microscopy is presented. The confocal microscope is configured with a horizontal light path, a 10 x dry objective, and an image‐intensified camera for collecting images at a magnification of...

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Veröffentlicht in:Scanning 1994-09, Vol.16 (5), p.316-319
Hauptverfasser: Mathers, William D., Daley, Thomas E.
Format: Artikel
Sprache:eng
Schlagworte:
confocal microscopy
Humans
Lipids - analysis
meibomian gland
Microscopy, Confocal - methods
tear film
tear lipid
Tears - chemistry
Tears - secretion
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Zusammenfassung:A new method for observing normal and pathologic states of the human tear film using tandem scanning confocal microscopy is presented. The confocal microscope is configured with a horizontal light path, a 10 x dry objective, and an image‐intensified camera for collecting images at a magnification of approximately 150x. The advantages of confocal microscopy can be used to collect reflected images of the human tear film with improved detail and resolution.
ISSN:0161-0457
1932-8745
DOI:10.1002/sca.4950160512