Transformation of Mollicutes with Single-Stranded Tn4001 DNA

Transformation of Mollicutes with Single-Stranded Tn4001 DNA

Acholeplasma oculi ISM1499 and Mycoplasma gallisepticum were transformed with single-stranded and double-stranded plasmids containing Tn4001. The transposon mobilized to the chromosome using both single-stranded and double-stranded DNA at the same frequency. M. gallisepticum transformed at a 2 log l...

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Veröffentlicht in:Plasmid 1994-07, Vol.32 (1), p.85-88
Hauptverfasser: Kapke, Paul A., Knudtson, Kevin L., Minion, F.Chris
Format: Artikel
Sprache:eng
Schlagworte:
Acholeplasma - genetics
DNA Transposable Elements - genetics
DNA, Bacterial - genetics
DNA, Single-Stranded - genetics
Models, Genetic
Mycoplasma - genetics
Transformation, Bacterial
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Zusammenfassung:Acholeplasma oculi ISM1499 and Mycoplasma gallisepticum were transformed with single-stranded and double-stranded plasmids containing Tn4001. The transposon mobilized to the chromosome using both single-stranded and double-stranded DNA at the same frequency. M. gallisepticum transformed at a 2 log lower frequency than did A. oculi ISM1499. Restriction enzyme digestion of single-stranded DNA indicated homologous base pairing in the inverted repeat regions, which could account for the transpositional activity of single-stranded DNA.
ISSN:0147-619X
1095-9890
DOI:10.1006/plas.1994.1048