The stereochemical course of group II intron self-splicing

The stereochemical specificities and reaction courses for both self-splicing steps of a group II intron have been determined by phosphorothioate substitution at the 5' and 3' splice site phosphodiester bonds. Both steps of the splicing reaction proceeded with a phosphorothioate in the Sp c...

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Veröffentlicht in:Science (American Association for the Advancement of Science) 1994-12, Vol.266 (5191), p.1685-1688
Hauptverfasser: Padgett, R.A. (University of Texas Southwestern Medical Ceter at Dallas, Dallas, TX.), Podar, M, Boulanger, S.C, Perlman, P.S
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Sprache:eng
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Zusammenfassung:The stereochemical specificities and reaction courses for both self-splicing steps of a group II intron have been determined by phosphorothioate substitution at the 5' and 3' splice site phosphodiester bonds. Both steps of the splicing reaction proceeded with a phosphorothioate in the Sp configuration but were blocked by the Rp diastereomer. Both steps also proceeded with inversion of stereochemical configuration around phosphonus, consistent with a concerted transesterification reaction. These results are identical to those found for nuclear precursor mRNA (pre-mRNA) splicing and provide support for the hypothesis that group II introns and nuclear pre-mRNA introns share a common evolutionary history
ISSN:0036-8075
1095-9203
DOI:10.1126/science.7527587