Determination of acid phosphatase in cultivation medium using asymmetrical flow field-flow fractionation

Determination of acid phosphatase in cultivation medium using asymmetrical flow field-flow fractionation

Highly glycosylated proteins and proteins larger than 100 000 Da are difficult to analyze in complex media with standard liquid chromatography techniques. Asymmetrical flow field-flow fractionation is a simple bio-compatible method for such analysis which can also be used for on-line monitoring. A m...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of biotechnology 1994-11, Vol.37 (3), p.291-295
Hauptverfasser: Litzén, A., Garn, M.B., Widmer, H.M.
Format: Artikel
Sprache:eng
Schlagworte:
Acid phosphatase
Acid Phosphatase - analysis
Acid Phosphatase - biosynthesis
Biological and medical sciences
Biotechnology
Chemical Fractionation - methods
Culture Media
Enzyme engineering
Fermentation
Fractionation
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Production of selected enzymes
Protein
Recombinant Proteins - analysis
S. cerevisiae
Saccharomyces cerevisiae
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Highly glycosylated proteins and proteins larger than 100 000 Da are difficult to analyze in complex media with standard liquid chromatography techniques. Asymmetrical flow field-flow fractionation is a simple bio-compatible method for such analysis which can also be used for on-line monitoring. A method for determination of acid phosphatase (EC 3.1.3.2), molecular weight 1 000 000, produced by a recombinant DNA yeast strain is presented.
ISSN:0168-1656
1873-4863
DOI:10.1016/0168-1656(94)90135-X