The Tumor Protein MAGE-1 Is Located in the Cytosol of Human Melanoma Cells

The Tumor Protein MAGE-1 Is Located in the Cytosol of Human Melanoma Cells

The MAGE-1 protein has been localized to the cytosol of human melanoma culture cells (MZ2-MEL.43) by subcellular fractionation. Its distribution between nuclear, mitochondrial, microsomal and cytosolic fractions was established by SDS-PAGE and immunoblotting with a rabbit antiserum and compared to t...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biochemical and biophysical research communications 1994-10, Vol.204 (2), p.710-715
Hauptverfasser: Amarcostesec, A., Godelaine, D., Stockert, E., Vanderbruggen, P., Beaufay, H., Chen, Y.T.
Format: Artikel
Sprache:eng
Schlagworte:
Antigens, Neoplasm - genetics
Antigens, Neoplasm - metabolism
Cytosol - metabolism
DNA, Complementary
Humans
Melanoma - metabolism
Melanoma-Specific Antigens
Microscopy, Fluorescence
Neoplasm Proteins
Protein Biosynthesis
Subcellular Fractions - metabolism
Transcription, Genetic
Tumor Cells, Cultured
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The MAGE-1 protein has been localized to the cytosol of human melanoma culture cells (MZ2-MEL.43) by subcellular fractionation. Its distribution between nuclear, mitochondrial, microsomal and cytosolic fractions was established by SDS-PAGE and immunoblotting with a rabbit antiserum and compared to that of markers for the main cell compartments. The immunoreactive material was recovered in the cytosolic fraction as a polypeptide migrating at 42 kDa, which has been further identified as the MAGE-1 protein because it comigrates with the product of cell-free transcription-translation of the MAGE-1 cDNA. Location to the cytosol was confirmed by immunofluorescence microscopy.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1994.2517