Effect of Prostaglandins on Interferon Synthesis in Murine Macrophage-like Cell Lines

Murine macrophage-like cell lines were used to determine whether exogenously added prostaglandins and endogenous prostaglandins suppress interferon (IFN) synthesis in macrophagesThe amount of IFN produced by J774A.1 cells induced with bacterial lipopolysaccharide (LPS) was reduced by 0.1 and 1 [tM PGE, or PGE2These prostaglandins also inhibited Newcastle disease virus (NDV) induced IFN production, but only at a concentration of 1 μMThromboxane B 2 at 0.01 to 1 μM had no effect on IFN productionCells treated before, during, or before and during IFN synthesis with 0.15 to 4.8 μM indomethacin to inhibit prostaglandin synthesis did not increase IFN yieldsIndomethacin also had no effect on NDV-induced IFN production by P388D 1 and PU5-1.8 cells, and these cells remained nonresponsive to LPS for IFN productionThese results indicate that endogenous levels of cyclooxygenase-dependent metabolites of arachidonic acid do not regulate IFN synthesis in macrophages.