Predicted sequence of the host-protective immunogen of infectious bursal disease virus

The genome of Australian strain 002-73 of infectious bursal disease virus (IBDV) has been cloned as cDNA fragments into an expression library based on pUR plasmid vectors. Recombinant colonies were selected with a monoclonal antibody specific for the 32-kDa host-protective immunogen of IBDV and full...

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Veröffentlicht in:	FEBS letters 1986-05, Vol.201 (1), p.143-146
Hauptverfasser:	Hudson, Peter J., McKern, Neil M., Fahey, Kevin J., Azad, Ahmed A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence Biological and medical sciences chickens DNA - genetics DNA, Recombinant Fundamental and applied biological sciences. Psychology GENBANK, the nucleotide sequence database of the Los Alamos National Laboratory, Los Alamos, USA Genes, Viral IBDV, infectious bursal disease virus Infectious bursal disease virus Infectious bursal disease virus - genetics infectious bursitis virus Mab, monoclonal antibody Microbiology monoclonal antibodies Morphology, structure, chemical composition, physicochemical properties NBRF, the protein sequence database of the National Biomedical Research Foundation, Washington, USA Nucleotide sequence Primary structure Reoviridae - genetics RNA, Viral - genetics Vaccine Viral Proteins - genetics Viral Proteins - immunology viral RNA Viral Vaccines Virology
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Zusammenfassung:	The genome of Australian strain 002-73 of infectious bursal disease virus (IBDV) has been cloned as cDNA fragments into an expression library based on pUR plasmid vectors. Recombinant colonies were selected with a monoclonal antibody specific for the 32-kDa host-protective immunogen of IBDV and fully characterized by nucleotide sequence analysis. The amino acid sequence of several tryptic peptides derived from the native 32-kDa structural protein has confirmed the coding region assignment and nucleotide sequence data. We believe this to be the first published sequence of a birnavirus-encoded protein and these data may provide the basis for an effective subunit vaccine against IBDV
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(86)80587-7