Inhibitory effects of histidine analogues on growth and protein synthesis by Plasmodium falciparum in vitro

The human malaria parasite Plasmodium falciparum synthesizes several proteins that are unusually rich in histidine. We therefore screened histidine analogues for their capacity to inhibit in vitro parasite growth. Analogues were added to cultures of ring-stage parasites, and parasite morphological development was assessed by light microscopy after a 22-hr culture. Inhibition of morphological development was identified as the appearance of condensed or pycnotic parasites rather than mature trophozoites. Inhibition of parasite protein synthesis was assessed by radioactivity counting of [ 3H] isoleucine incorporated into acid-insoluble products and by sodium dodecyl sulfate polyacrylamide gel electrophoresis and fluorography of [ 3H]histidine-labeled malarial proteins. 2-F- l-Histidine and 2-I- d, L-histidine exerted the most pronounced inhibitory effects, the fluoro-analogue being the more effective of the two. At a 0.125 mM concentration, both compounds inhibited parasite growth and 2-F- l-histidine also inhibited protein synthesis. At a 1.0 mM concentration, 2-azido- l-histidine, α-methyl- l-histidine and WR 177589A also inhibited P. falciparum growth and protein synthesis. Twenty other histidine analogues, including 5-F- l-histidine and 5-I- l-histidine, showed little or no effect under these conditions. The inhibitory histidine analogues may be of interest for antimalarial chemotherapy it they should prove to have greater effect on P. falciparum protein synthesis than on host protein synthesis.