A simple and rapid chemical method for the determination of cephalosporins

A simple and rapid chemical method for the determination of cephalosporins

A simple and rapid chemical assay for cephalosporins is described. It is a simple modification of the colorimetric determination of penicillins in which the narrow spectrum beta-lactamase (penicillinase) is replaced by a broad spectrum beta-lactamase (cephalosporinase) produced by Enterobacter cloac...

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Veröffentlicht in:Applied biochemistry and biotechnology 1986-02, Vol.12 (1), p.31-35
Hauptverfasser: Lübbe, C, Shen, Y Q, Demain, A L
Format: Artikel
Sprache:eng
Schlagworte:
Cephalosporinase - metabolism
Cephalosporins - analysis
Colorimetry - methods
Indicators and Reagents
Kinetics
Molybdenum
Penicillinase
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Beschreibung
Zusammenfassung:A simple and rapid chemical assay for cephalosporins is described. It is a simple modification of the colorimetric determination of penicillins in which the narrow spectrum beta-lactamase (penicillinase) is replaced by a broad spectrum beta-lactamase (cephalosporinase) produced by Enterobacter cloacae. The method can be used for assay of fermentation broths as well as for pure cephalosporins.
ISSN:0273-2289
1559-0291
DOI:10.1007/BF02798577