Identification of a cellular transcription factor involved in E1A trans-activation

We have employed a gel assay to detect a transcription factor in nuclear extracts of adenovirus-infected cells that interacts with the adenovirus E2 promoter, an E1A inducible promoter. Binding of the factor to the promoter protected sequences between −33 and −74 from DNAase cleavage in a footprint...

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Veröffentlicht in:Cell 1986-04, Vol.45 (2), p.219-228
Hauptverfasser: Kovesdi, Imre, Reichel, Ronald, Nevins, Joseph R.
Format: Artikel
Sprache:eng
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Zusammenfassung:We have employed a gel assay to detect a transcription factor in nuclear extracts of adenovirus-infected cells that interacts with the adenovirus E2 promoter, an E1A inducible promoter. Binding of the factor to the promoter protected sequences between −33 and −74 from DNAase cleavage in a footprint assay. This region was also protected from exonuclease III digestion. These sequences coincide with those previously shown to be essential for promoter activity and E1A stimulation. The factor could be detected in extracts of uninfected cells, although at greatly reduced levels. The increased level of factor in infected cells required a functional E1A gene. These results suggest that the E2 binding activity is a cellular transcriptional factor, the concentration or binding activity of which increases as a result of the action of the E1A gene product.
ISSN:0092-8674
1097-4172
DOI:10.1016/0092-8674(86)90386-7