Separation of 24 dansylamino acids by capillary electrophoresis with a non-ionic surfactant

The separation of 24 dansylamino acids was investigated by capillary electrophoresis with an additive of micelles of a non-ionic surfactant, Tween 20. Although two pairs of peaks, norvaline and methionine derivatives, and didansyltyrosine and solvent (methanol), did not show good resolution, other d...

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Veröffentlicht in:Journal of Chromatography A 1994-09, Vol.680 (1), p.311-315
Hauptverfasser: Matsubara, Norio, Terabe, Shigeru
Format: Artikel
Sprache:eng
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Zusammenfassung:The separation of 24 dansylamino acids was investigated by capillary electrophoresis with an additive of micelles of a non-ionic surfactant, Tween 20. Although two pairs of peaks, norvaline and methionine derivatives, and didansyltyrosine and solvent (methanol), did not show good resolution, other dansylamino acids were well separated within 70 min using 100 m M Tween 20 and pH 2.40. The theoretical plate numbers calculated for dansylamino acids were 28 000–111 000 with a 19-cm capillary column.
ISSN:0021-9673
DOI:10.1016/0021-9673(94)80081-2