Direct observation of tryptophan biosynthesis in Escherichia coli by carbon-13 nuclear magnetic resonance spectroscopy
Carbon-13 nuclear magnetic resonance (NMR) spectroscopy has been applied to the direct monitoring of L-tryptophan biosynthesis in genetically modified E. coli . To monitor tryptophan production in a fermentor, a device was constructed that allowed the continuous pumping of ferment directly into and...
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| Veröffentlicht in: | Analytical chemistry (Washington) 1986-03, Vol.58 (3), p.526-532 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Ashworth, Dennis J Chen, Chi S Mascarenhas, Desmond |
| description | Carbon-13 nuclear magnetic resonance (NMR) spectroscopy has been applied to the direct monitoring of L-tryptophan biosynthesis in genetically modified E. coli . To monitor tryptophan production in a fermentor, a device was constructed that allowed the continuous pumping of ferment directly into and out of a special NMR tube while growth of the culture was maintained. |
| doi_str_mv | 10.1021/ac00294a006 |
| format | Article |
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To monitor tryptophan production in a fermentor, a device was constructed that allowed the continuous pumping of ferment directly into and out of a special NMR tube while growth of the culture was maintained.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac00294a006</identifier><identifier>PMID: 3083717</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Aminoacids, peptides. Hormones. Neuropeptides ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Carbon Isotopes ; Escherichia coli ; Escherichia coli - metabolism ; Fundamental and applied biological sciences. 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Psychology</subject><subject>Glucose - metabolism</subject><subject>Glycine - metabolism</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Proteins</subject><subject>Serine - metabolism</subject><subject>Tryptophan - biosynthesis</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U2LFDEQBuAgyjq7evIs5CB6kNYk_ZHkuKy7frDigqMHL6GSSTtZe5I21b3Y_97IDIMHwVMg9VBJvUXIE85ecSb4a3CMCd0AY909suKtYFWnlLhPVoyxuhKSsYfkFPGWMc4Z707ISc1ULblckbs3IXs30WTR5zuYQoo09XTKyzilcQuR2pBwidPWY0AaIr1Et_U5uG0A6tIQqF2og2xTrHhN4-wGD5nu4Hv0U3A0e0wRovMUx_JQTujSuDwiD3oY0D8-nGfky9Xl-uJddf3p7fuL8-sK6q6dKhC9k63iPe-0tpqVS7vZlAn1RjvVtEo55XvZQyuctkrapuFSCi88tFZoXp-R5_u-Y04_Z4-T2QV0fhgg-jSjkZ1UWtbqv5A3rVYl7AJf7qEro2D2vRlz2EFeDGfmzzrMX-so-umh7Wx3fnO0h_xL_dmhDuhg6HNJKuCRSd00LW8Kq_Ys4OR_HcuQf5hO1rI165vPpv6w_vb1483asOJf7D04NLdpzrGE_M8P_gZ2_67Q</recordid><startdate>198603</startdate><enddate>198603</enddate><creator>Ashworth, Dennis J</creator><creator>Chen, Chi S</creator><creator>Mascarenhas, Desmond</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>198603</creationdate><title>Direct observation of tryptophan biosynthesis in Escherichia coli by carbon-13 nuclear magnetic resonance spectroscopy</title><author>Ashworth, Dennis J ; Chen, Chi S ; Mascarenhas, Desmond</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a365t-a2fc7581f1699b90365bdd1529d9c84588c8ef7fa52c9b87b441772e2ea5b2913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Aminoacids, peptides. Hormones. Neuropeptides</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Carbon Isotopes</topic><topic>Escherichia coli</topic><topic>Escherichia coli - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose - metabolism</topic><topic>Glycine - metabolism</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Proteins</topic><topic>Serine - metabolism</topic><topic>Tryptophan - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ashworth, Dennis J</creatorcontrib><creatorcontrib>Chen, Chi S</creatorcontrib><creatorcontrib>Mascarenhas, Desmond</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ashworth, Dennis J</au><au>Chen, Chi S</au><au>Mascarenhas, Desmond</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct observation of tryptophan biosynthesis in Escherichia coli by carbon-13 nuclear magnetic resonance spectroscopy</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. 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| fulltext | fulltext |
| identifier | ISSN: 0003-2700 |
| ispartof | Analytical chemistry (Washington), 1986-03, Vol.58 (3), p.526-532 |
| issn | 0003-2700 1520-6882 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76789738 |
| source | MEDLINE; ACS Publications |
| subjects | Aminoacids, peptides. Hormones. Neuropeptides Analytical, structural and metabolic biochemistry Biological and medical sciences Carbon Isotopes Escherichia coli Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology Glucose - metabolism Glycine - metabolism Magnetic Resonance Spectroscopy Proteins Serine - metabolism Tryptophan - biosynthesis |
| title | Direct observation of tryptophan biosynthesis in Escherichia coli by carbon-13 nuclear magnetic resonance spectroscopy |
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