Direct observation of tryptophan biosynthesis in Escherichia coli by carbon-13 nuclear magnetic resonance spectroscopy

Carbon-13 nuclear magnetic resonance (NMR) spectroscopy has been applied to the direct monitoring of L-tryptophan biosynthesis in genetically modified E. coli . To monitor tryptophan production in a fermentor, a device was constructed that allowed the continuous pumping of ferment directly into and...

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Veröffentlicht in:	Analytical chemistry (Washington) 1986-03, Vol.58 (3), p.526-532
Hauptverfasser:	Ashworth, Dennis J, Chen, Chi S, Mascarenhas, Desmond
Format:	Artikel
Sprache:	eng
Schlagworte:	Aminoacids, peptides. Hormones. Neuropeptides Analytical, structural and metabolic biochemistry Biological and medical sciences Carbon Isotopes Escherichia coli Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology Glucose - metabolism Glycine - metabolism Magnetic Resonance Spectroscopy Proteins Serine - metabolism Tryptophan - biosynthesis
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Beschreibung
Zusammenfassung:	Carbon-13 nuclear magnetic resonance (NMR) spectroscopy has been applied to the direct monitoring of L-tryptophan biosynthesis in genetically modified E. coli . To monitor tryptophan production in a fermentor, a device was constructed that allowed the continuous pumping of ferment directly into and out of a special NMR tube while growth of the culture was maintained.
ISSN:	0003-2700 1520-6882
DOI:	10.1021/ac00294a006