The two-hybrid system: an assay for protein-protein interactions

The two-hybrid system: an assay for protein-protein interactions

The two-hybrid system is a yeast-based genetic assay for detecting protein-protein interactions. It can be used to identify proteins that bind to a protein of interest, or to delineate domains or residues critical for an interaction. Variations on this methodology have been developed to clone genes...

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Veröffentlicht in:Trends in Genetics 1994-08, Vol.10 (8), p.286-292
Hauptverfasser: Fields, Stanley, Sternglanz, Rolf
Format: Artikel
Sprache:eng
Schlagworte:
DNA-Binding Proteins - metabolism
Escherichia coli
Protein Binding
Proteins - metabolism
Saccharomyces cerevisiae
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Beschreibung
Zusammenfassung:The two-hybrid system is a yeast-based genetic assay for detecting protein-protein interactions. It can be used to identify proteins that bind to a protein of interest, or to delineate domains or residues critical for an interaction. Variations on this methodology have been developed to clone genes that encode DNA-binding proteins, to identify peptides that bind to a protein and, potentially, to screen for drugs.
ISSN:0168-9525
DOI:10.1016/0168-9525(90)90012-U