Tissue factor activity in hela cells measured with a continuous chromogenic assay and elisa reader

Tissue factor activity in hela cells measured with a continuous chromogenic assay and elisa reader

Tissue factor activity expressed by Hela cells cultured in 96-well plates has been quantitated in situ using a continuous spectrophotometric assay. Following the assay, cells assayed without physical disruption remained as viable as cells not subjected to the assay. Very little (or no) tissue factor...

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Veröffentlicht in:Thrombosis research 1986-01, Vol.41 (2), p.185-195
Hauptverfasser: Carson, Steven D., Archer, Philip G.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Biological and medical sciences
Blood coagulation. Blood cells
Cattle
cell culture
cell density
chromogenic assay
Cytological Techniques
Enzyme-Linked Immunosorbent Assay
Factor VII - physiology
Factor VIIa
Factor X - physiology
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods, hemostasis, fibrinolysis
HeLa Cells - metabolism
Humans
Methods
Models, Biological
Molecular and cellular biology
Spectrophotometry
Thromboplastin - metabolism
Time Factors
tissue factor
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Zusammenfassung:Tissue factor activity expressed by Hela cells cultured in 96-well plates has been quantitated in situ using a continuous spectrophotometric assay. Following the assay, cells assayed without physical disruption remained as viable as cells not subjected to the assay. Very little (or no) tissue factor was expressed in nondisrupted cells relative to that available in cells disrupted by freeze-thawing and sonication. Total tissue factor activity (that available in disrupted cells) decreased not as a simple function of time after subculturing, but was inversely related to cell density.
ISSN:0049-3848
1879-2472
DOI:10.1016/0049-3848(86)90228-8