Purification of a Cytotoxic Protein Produced by the Murine Macrophage-Like Cell Line J774.1 in Response to Sarcophaga Lectin

A tumor specific cytotoxic protein produced by the murine macrophage-like cell line J774.1 in response to stimulation with Sarcophaga lectin was purified to homogeneity in three steps from the culture medium. This cytotoxin, named tumor killing factor (TKF), was a protein with a molecular weight of...

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Veröffentlicht in:	Journal of biochemistry (Tokyo) 1986-01, Vol.99 (1), p.9-15
Hauptverfasser:	ITOH, Akira, OHSAWA, Fukuichi, NATORI, Shunji
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids - analysis Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cell Line Chemical Phenomena Chemistry cytotoxins Diptera Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Glycoproteins - biosynthesis Glycoproteins - isolation & purification Insect Proteins lectins Lectins - pharmacology Lectins, C-Type macrophages Macrophages - metabolism Mice Miscellaneous Molecular Weight Proteins Sarcophaga Tumor Necrosis Factor-alpha
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Zusammenfassung:	A tumor specific cytotoxic protein produced by the murine macrophage-like cell line J774.1 in response to stimulation with Sarcophaga lectin was purified to homogeneity in three steps from the culture medium. This cytotoxin, named tumor killing factor (TKF), was a protein with a molecular weight of 15,000, and aggregated forming an oligomer with a molecular weight of 48,000. Its amino acid composition was similar to that of human TNF. Purified TKF had a significant effect on transplanted murine ascites tumor sarcoma 180. The biological significance of TKF in terms of ontogeny is discussed from the view point of developmental biology.
ISSN:	0021-924X 1756-2651
DOI:	10.1093/oxfordjournals.jbchem.a135483