Universal β-galactosidase cloning vectors for promoter analysis and gene targeting

Two new plasmid vectors suitable for generating fusions with the lacZ gene have been developed and tested. The vectors can be applied in the analysis of regulatory elements of eukaryotic genes in both transient and stable transfection experiments. In addition, they can be utilized as the backbone of...

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Veröffentlicht in:	Gene 1994-10, Vol.148 (1), p.67-70
Hauptverfasser:	Kaestner, Klaus H., Montoliu, Lluís, Kern, Heidrun, Thulke, Monika, Schütz, Günther
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence beta-Galactosidase - genetics Cloning, Molecular Gene Expression gene fusions Gene Targeting - methods Genes, Reporter Genetic Vectors homologous recombination Humans lacZ Molecular Sequence Data neomycin phosphotransferase Promoter Regions, Genetic - genetics Recombinant DNA Transfection Tumor Cells, Cultured
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Beschreibung
Zusammenfassung:	Two new plasmid vectors suitable for generating fusions with the lacZ gene have been developed and tested. The vectors can be applied in the analysis of regulatory elements of eukaryotic genes in both transient and stable transfection experiments. In addition, they can be utilized as the backbone of gene targeting vectors, allowing the assessment of the expression pattern of the targeted gene by staining for β-galactosidase activity.
ISSN:	0378-1119 1879-0038
DOI:	10.1016/0378-1119(94)90234-8