Cloning of a somatolactin-encoding cDNA from sole ( Solea senegalensis)

From a Solea senegalensis cDNA expression library, clones encoding somatolactin (SL), a new pituitary hormone belonging to the growth hormone/prolactin family, were isolated and analyzed. Northern blot analysis showed a unique 1.0-kb mRNA species. The sole SL 778-bp cDNA encoded full-size S. senegal...

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Veröffentlicht in:	Gene 1994-09, Vol.147 (2), p.227-230
Hauptverfasser:	Pendón, Carlos, Martinez-Barberá, Juan Pedro, Valdivia, Manuel M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence amino-acid sequence homology Animals Base Sequence Blotting, Northern Cloning, Molecular DNA, Complementary fish Fish Proteins Flatfishes - genetics Glycoproteins - genetics Marine Molecular Sequence Data nucleotide sequence homology Pituitary Pituitary Hormones - genetics Sequence Homology, Amino Acid Solea senegalensis
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Beschreibung
Zusammenfassung:	From a Solea senegalensis cDNA expression library, clones encoding somatolactin (SL), a new pituitary hormone belonging to the growth hormone/prolactin family, were isolated and analyzed. Northern blot analysis showed a unique 1.0-kb mRNA species. The sole SL 778-bp cDNA encoded full-size S. senegalensis SL (ssSL) (230 amino acids), including seven Cys and two potential glycosylation sites. A consensus polyadenylation signal, AATAAA, was found. Protein homology and DNA sequence alignments of SL cDNAs from other evolutionarily distant marine fishes suggest that the SL sequence is highly conserved.
ISSN:	0378-1119 1879-0038
DOI:	10.1016/0378-1119(94)90071-X