The increase in gene expression induced by introduction of rare codons into the C terminus of the template

Short oligodeoxynucleotides (oligos) possessing two tandem Arg codons followed by TGA stop codon were inserted near the 3′ end of a modified cat gene. It was found that while being decoded in vivo, the AGGAGGTGA oligo increased the yield of gene product and, in addition, caused − 1 frameshifting. Th...

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Veröffentlicht in:Gene 1994-10, Vol.148 (1), p.15-21
Hauptverfasser: Gursky, Yaroslav G., Sh.Beabealashvilli, Robert
Format: Artikel
Sprache:eng
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Zusammenfassung:Short oligodeoxynucleotides (oligos) possessing two tandem Arg codons followed by TGA stop codon were inserted near the 3′ end of a modified cat gene. It was found that while being decoded in vivo, the AGGAGGTGA oligo increased the yield of gene product and, in addition, caused − 1 frameshifting. The 3-10-fold increase of the yield of the polypeptide was accompanied by increased accumulation of corresponding mRNA, indicating a protection from messenger decay. Transformation of the cells by a plasmid overproducing tRNA Arg 4 gene compensates for all the anomalies.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(94)90228-3