A simple high-performance liquid chromatographic method for estimating human serum angiotensin-converting enzyme activity

A simple method for measuring angiotensin-converting enzyme activity in human serum was developed. Samples were incubated with hippurylhistidylleucine and the liberated hippuric acid was determined directly by reversed phase ion-pair high-performance liquid chromatography with UV spectrometric detec...

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Veröffentlicht in:Journal of pharmaceutical and biomedical analysis 1985, Vol.3 (1), p.51-57
Hauptverfasser: Maurich, V., Moneghini, M., Pitotti, A., Vio, L.
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container_issue 1
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container_title Journal of pharmaceutical and biomedical analysis
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creator Maurich, V.
Moneghini, M.
Pitotti, A.
Vio, L.
description A simple method for measuring angiotensin-converting enzyme activity in human serum was developed. Samples were incubated with hippurylhistidylleucine and the liberated hippuric acid was determined directly by reversed phase ion-pair high-performance liquid chromatography with UV spectrometric detection.
doi_str_mv 10.1016/0731-7085(85)80006-6
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subjects Analytical, structural and metabolic biochemistry
angiotensin-converting enzyme assay
Biological and medical sciences
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
hippuric acid determination
Hydrolases
Ion-pair high-performance liquid chromatography
title A simple high-performance liquid chromatographic method for estimating human serum angiotensin-converting enzyme activity
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